Kıvanç Sertaç Argun, Kıvanç Merih, Kılıç Volkan, Güllülü Gülay, Özmen Ahmet Tuncer
Uludağ University Faculty of Medicine, Department of Ophthalmology, Bursa, Turkey.
Anadolu University Faculty of Science, Department of Biology, Eskişehir, Turkey.
Turk J Ophthalmol. 2017 Apr;47(2):68-73. doi: 10.4274/tjo.79059. Epub 2017 Apr 1.
To compare biofilm formations of two isolates with known biofilm formation capacities on four different intraocular lenses (IOL) that have not been studied before.
Two isolates obtained from ocular surfaces and identified in previous studies and stored at -86 °C in 15% glycerol in the microbiology laboratory of the Anadolu University Department of Biology were purified and used in the study. The isolates were KA 15.8 (ICA+), a known biofilm producer isolate positive for and genes, and KA 14.5 (ICA-), known as a non-biofilm producer isolate negative for and genes. The biofilm formation capacities of the 2 isolates on 4 different IOLs were compared. Two of the IOLs were acrylic (UD613 [IOL A], Turkey; SA60AT [IOL B], USA), and the other two were polymethyl methacrylate (PMMA) (B60130C [IOL C], India; B55125C [IOL D], India). Bacterial enumeration and optical density measurements were done from biofilms that formed on the IOLs. Biofilms were imaged using scanning electron microscopy.
Mean bacterial counts on the IOLs were 7.1±0.4 log CFU/mL with the ICA+ isolate, and 6.7±0.8 log CFU/mL with the ICA- isolate; there were no statistically significant differences. Biofilm formation was lower with acrylic lenses than PMMA lenses with both isolates (p=0.009 and p=0.013). The highest biofilm production was obtained on IOL C (PMMA) (p<0.001) and the lowest was obtained on IOL A (hydrophilic acrylic) (p<0.001).
Bacterial counts after biofilm formation were lower on acrylic lenses, especially hydrophilic acrylic with hydrophobic properties. Further animal and studies are required to support the findings of this study.
比较两种已知具有生物膜形成能力的菌株在四种此前未被研究过的不同人工晶状体（IOL）上的生物膜形成情况。
从眼表获取并在先前研究中鉴定的两种菌株保存在阿纳多卢大学生物学系微生物实验室的-86°C、含15%甘油的环境中，将其纯化后用于本研究。这两种菌株分别是KA 15.8（ICA+），一种已知的生物膜产生菌，对 和 基因呈阳性，以及KA 14.5（ICA-），一种已知的非生物膜产生菌，对 和 基因呈阴性。比较了这两种菌株在4种不同人工晶状体上的生物膜形成能力。其中两种人工晶状体是丙烯酸酯材质（UD613 [IOL A]，土耳其；SA60AT [IOL B]，美国），另外两种是聚甲基丙烯酸甲酯（PMMA）材质（B60130C [IOL C]，印度；B55125C [IOL D]，印度）。对在人工晶状体上形成的生物膜进行细菌计数和光密度测量。使用扫描电子显微镜对生物膜进行成像。
ICA+菌株在人工晶状体上的平均细菌计数为7.1±0.4 log CFU/mL，ICA-菌株为6.7±0.8 log CFU/mL；两者无统计学显著差异。两种菌株在丙烯酸酯晶状体上的生物膜形成均低于PMMA晶状体（p = 0.009和p = 0.013）。在IOL C（PMMA）上获得的生物膜产量最高（p<0.001），在IOL A（亲水性丙烯酸酯）上获得的最低（p<0.001）。
生物膜形成后的细菌计数在丙烯酸酯晶状体上较低，尤其是具有疏水特性的亲水性丙烯酸酯。需要进一步的动物和 研究来支持本研究的结果。
