Ryu H W, Kang K H, Pan J G, Chang H N
Department of Biochemical Engineering, Chonnam National University, Kwangju 500-757, Korea.
Biotechnol Bioeng. 2001 Jan 5;72(1):119-24. doi: 10.1002/1097-0290(20010105)72:1<119::aid-bit15>3.0.co;2-0.
Enterococcus faecalis RKY1, which converts fumarate to succinate with a high yield, was identified on the basis of a phylogenetic analysis of the 16S rDNA gene sequence. The strain was incubated at 38 degrees C for 18 h to examine the possible diversion of glucose or glycerol fermentation by fumarate. The products of glucose and glycerol fermentation with fumarate were quite different from those of normal fermentation, which ultimately produces lactate, in that mainly succinate is produced. Metabolic pathway stoichiometry was used to analyze the oxidation of glycerol to succinate by Enterococcus faecalis RKY1. The stoichiometric relationship between glycerol and fumarate was used as a guideline to accumulate succinate more efficiently.
粪肠球菌RKY1能够高产率地将富马酸盐转化为琥珀酸盐，该菌株是基于16S rDNA基因序列的系统发育分析鉴定出来的。将该菌株在38℃下培养18小时，以研究富马酸盐是否可能改变葡萄糖或甘油的发酵途径。在有富马酸盐存在的情况下，葡萄糖和甘油的发酵产物与正常发酵最终产生乳酸的产物有很大不同，主要产生的是琥珀酸盐。利用代谢途径化学计量学分析了粪肠球菌RKY1将甘油氧化为琥珀酸盐的过程。甘油与富马酸盐之间的化学计量关系被用作更有效地积累琥珀酸盐的指导原则。
