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[通过免疫荧光法在自身免疫性疾病中鉴定抗体]

[Identification of antibodies by means of immunofluorescence in autoimmune diseases].

作者信息

Storch W

出版信息

Z Gesamte Inn Med. 1975 Nov 15;30(22):245-8.

PMID:1108470
Abstract

A short survey of the methods of the immunofluorescence microscopy for the demonstration of various humoral antibodies is given. It is referred to the necessity of a far-reaching standardisation, whereby especially exact data in publications are necessary. Apart from the fluorochromium/protein ratio as conjuncate criteria among others have proved the ratio of antibody protein to the total protein content and the suitability test at the antigenic material concerned. The following antibodies are dealt with in particulars; antinuclear factors, antibodies of thrombocytes, antibodies of the skeletal muscles, antibodies against smooth musculature, antibodies against mitochondria, antibodies of parietal cells, antibodies against microsomas and antibodies against structures of connective tissue.

摘要

本文简要介绍了用于检测各种体液抗体的免疫荧光显微镜方法。文中提到了进行广泛标准化的必要性,因此出版物中需要特别准确的数据。除了荧光染料/蛋白质比例作为结合标准外,抗体蛋白与总蛋白含量的比例以及针对相关抗原物质的适用性测试也已得到证实。本文详细讨论了以下抗体:抗核因子、血小板抗体、骨骼肌抗体、平滑肌抗体、线粒体抗体、壁细胞抗体、微粒体抗体和结缔组织结构抗体。

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