Wei N R, Giebel W
Laryngol Rhinol Otol (Stuttg). 1987 Jan;66(1):6-10.
Antibodies in the serum of patients with inner ear diseases can be demonstrated immunohistochemically with human kidney tissue. In this study a patient's serum with the typical positive reaction against proximal tubuli (antibodies against microsomes) and a serum with antibodies against mitochondria was tested with different dilutions of the fluorescein marked antiserum (FITC conjugate). For both sera a dilution of 1:160 showed positive reaction. The fluorescence of the control incubation was negligible. Employing the given technical details the immunohistochemical assay of antibodies with human kidney tissue has become a simple and reliable test for the most frequently found antibodies in patients with inner ear diseases.
内耳疾病患者血清中的抗体可用人肾组织进行免疫组织化学检测。在本研究中，用不同稀释度的荧光素标记抗血清（FITC 偶联物）检测了一份对近端小管呈典型阳性反应的患者血清（抗微粒体抗体）和一份抗线粒体抗体血清。两份血清在 1:160 的稀释度下均显示阳性反应。对照孵育的荧光可忽略不计。采用给定的技术细节，用人肾组织进行抗体的免疫组织化学检测已成为检测内耳疾病患者中最常见抗体的一种简单可靠的方法。
