Feng G, Mellor R H, Bernstein M, Keller-Peck C, Nguyen Q T, Wallace M, Nerbonne J M, Lichtman J W, Sanes J R
Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri 63110, USA.
Neuron. 2000 Oct;28(1):41-51. doi: 10.1016/s0896-6273(00)00084-2.
We generated transgenic mice in which red, green, yellow, or cyan fluorescent proteins (together termed XFPs) were selectively expressed in neurons. All four XFPs labeled neurons in their entirety, including axons, nerve terminals, dendrites, and dendritic spines. Remarkably, each of 25 independently generated transgenic lines expressed XFP in a unique pattern, even though all incorporated identical regulatory elements (from the thyl gene). For example, all retinal ganglion cells or many cortical neurons were XFP positive in some lines, whereas only a few ganglion cells or only layer 5 cortical pyramids were labeled in others. In some lines, intense labeling of small neuronal subsets provided a Golgi-like vital stain. In double transgenic mice expressing two different XFPs, it was possible to differentially label 3 neuronal subsets in a single animal.
我们培育了转基因小鼠，其中红色、绿色、黄色或青色荧光蛋白（统称为XFP）在神经元中选择性表达。所有这四种XFP都能完整地标记神经元，包括轴突、神经末梢、树突和树突棘。值得注意的是，25个独立产生的转基因品系中的每一个都以独特的模式表达XFP，尽管它们都整合了相同的调控元件（来自thy1基因）。例如，在一些品系中，所有视网膜神经节细胞或许多皮质神经元都是XFP阳性的，而在其他品系中，只有少数神经节细胞或只有第5层皮质锥体神经元被标记。在一些品系中，对小神经元亚群的强烈标记提供了一种类似高尔基染色的活体染色。在表达两种不同XFP的双转基因小鼠中，有可能在单个动物中对3个神经元亚群进行差异标记。
