Walsh Mark K, Quigley Harry A
Wilmer Eye Institute, Johns Hopkins University School of Medicine, 600 N. Wolfe Street, Baltimore, MD 21287, USA.
J Neurosci Methods. 2008 Mar 30;169(1):214-21. doi: 10.1016/j.jneumeth.2007.11.029. Epub 2007 Dec 8.
We have developed a technique that permits time-lapse imaging of retinal ganglion cells (RGCs), their dendritic arbors and their axons in mammals in vivo. This technique utilizes a standard confocal laser scanning microscope, transgenic mice that express yellow fluorescent protein (YFP) in a subset of RGCs and survival anesthesia techniques. The same individual RGCs with their dendritic arbors and axons were multiply imaged in vivo in both adult and juvenile mice. Additionally, the same RGC that was imaged in vivo could then be located and imaged in fixed retinal whole mount preparations. This novel technique has many potential applications.
我们已经开发出一种技术，该技术能够对哺乳动物体内的视网膜神经节细胞（RGCs）、其树突分支及其轴突进行延时成像。这项技术利用标准的共聚焦激光扫描显微镜、在一部分RGCs中表达黄色荧光蛋白（YFP）的转基因小鼠以及存活麻醉技术。在成年和幼年小鼠体内对具有树突分支和轴突的相同单个RGCs进行了多次成像。此外，在体内成像的同一个RGC随后可以在固定的视网膜整装标本中定位并成像。这项新技术有许多潜在的应用。
