Gupta S K, Pillarisetti K, Ohlstein E H
Department of Cardiovascular Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania 19406, USA.
IUBMB Life. 2000 Jul;50(1):51-6. doi: 10.1080/15216540050176593.
The stimulatory mAb F11 binds two platelet membrane proteins of 32 and 35 kDa and causes activation of platelets when cross-linked with the FcgammaRII receptor. We used bioinformatics to identify expressed sequence tags from libraries of cytokine-stimulated human endothelial cell (EC) cDNAs. The protein sequence deduced from full-length F11 cDNA was identical to partial sequences of peptides derived from affinity-purified platelet F11 antigen. F11 mRNA is expressed in human EC, macrophages, and a variety of non-hematopoietic vascular tissues. Expression of F11 mRNA is modulated by cytokines in EC and is up-regulated by oxidized low-density lipoprotein in human macrophages. The F11 receptor contains two immunoglobulin-like domains in its 236-amino-acid-long extracellular region, and has identity to the recently described junctional adhesion molecule. The data indicate that the F11 antigen is a novel receptor or cell adhesion molecule belonging to the immunoglobulin superfamily.
刺激性单克隆抗体F11可结合两种分子量分别为32 kDa和35 kDa的血小板膜蛋白，当与FcγRII受体交联时可导致血小板活化。我们利用生物信息学从细胞因子刺激的人内皮细胞（EC）cDNA文库中鉴定表达序列标签。从全长F11 cDNA推导的蛋白质序列与源自亲和纯化的血小板F11抗原的肽的部分序列相同。F11 mRNA在人内皮细胞、巨噬细胞和多种非造血血管组织中表达。F11 mRNA的表达在EC中受细胞因子调节，在人巨噬细胞中被氧化型低密度脂蛋白上调。F11受体在其236个氨基酸长的细胞外区域含有两个免疫球蛋白样结构域，与最近描述的连接粘附分子具有同源性。数据表明F11抗原是一种属于免疫球蛋白超家族的新型受体或细胞粘附分子。
