Bourne N, Schleiss M R, Bravo F J, Bernstein D I
Children's Hospital Medical Center, Division of Infectious Diseases, Cincinnati, Ohio 45229, USA.
J Infect Dis. 2001 Jan 1;183(1):59-64. doi: 10.1086/317654. Epub 2000 Nov 21.
The guinea pig (gp) model of congenital cytomegalovirus (CMV) infection was used to evaluate a gpCMV glycoprotein vaccine. Hartley guinea pigs were immunized 3 times with 50 microg of lectin column-purified glycoproteins prepared from gpCMV-infected or -uninfected tissue culture. Immunization with the gpCMV vaccine produced seroconversion in all animals. Animals then were placed with gpCMV-seronegative male animals and were challenged late in pregnancy with virulent salivary gland-passaged gpCMV. Immunization with gpCMV glycoproteins significantly improved pregnancy outcome, with 54 of 63 pups live-born in immunized animals, compared with 21 of 48 in the controls (P<.001). In addition, virus was isolated from 24 of 54 live-born pups born to immunized mothers, compared with 16 of 20 live-born pups born to controls, indicating that immunization significantly reduced in utero transmission in surviving animals (P<.01).
采用先天性巨细胞病毒（CMV）感染的豚鼠模型来评估豚鼠CMV糖蛋白疫苗。用从感染或未感染豚鼠CMV的组织培养物中制备的50微克经凝集素柱纯化的糖蛋白对Hartley豚鼠进行3次免疫。用豚鼠CMV疫苗免疫可使所有动物产生血清转化。然后将这些动物与豚鼠CMV血清阴性的雄性动物放在一起，并在妊娠后期用强毒株唾液腺传代的豚鼠CMV进行攻击。用豚鼠CMV糖蛋白免疫显著改善了妊娠结局，免疫组63只幼崽中有54只存活出生，而对照组48只幼崽中只有21只存活出生（P<0.001）。此外，免疫组母亲所生的54只存活出生幼崽中有24只分离出病毒，而对照组所生的20只存活出生幼崽中有16只分离出病毒，这表明免疫显著降低了存活动物的子宫内传播（P<0.01）。
