Suwa T, Hogg J C, English D, Van Eeden S F
Pulmonary Research Laboratory, University of British Columbia, St. Paul's Hospital, Vancouver, British Columbia, Canada V6Z 1Y6.
Am J Physiol Heart Circ Physiol. 2000 Dec;279(6):H2954-60. doi: 10.1152/ajpheart.2000.279.6.H2954.
Recombinant human interleukin-6 (IL-6) causes both a thrombocytosis and leukocytosis. The thrombocytosis is caused by an accelerating thrombocytopoiesis, but the mechanism of the leukocytosis is unknown. This study was designed to determine the relative contributions of marrow stimulation and intravascular demargination to the IL-6 induced neutrophilia. IL-6 (2 microgram/kg), administered intravenously to rabbits, caused a biphasic neutrophilia with an initial peak at 3 h and a second peak at 9 h. Using the thymidine analog 5'-bromo-2'-deoxyuridine (BrdU) to label dividing polymorphonuclear leukocytes (PMNs) in the bone marrow, we showed that IL-6 treatment mobilizes PMNs from the marginated pool into the circulating pool at 2-6 h with a decrease in L-selectin expression on PMNs and also accelerates the release of PMNs from the postmitotic pool in the bone marrow at 12-24 h. We have concluded that IL-6 causes a biphasic neutrophilia wherein the first peak results from the mobilization of PMNs into the circulating pool from the marginated pool and the second peak results from an accelerated bone marrow release of PMNs.
重组人白细胞介素-6（IL-6）可引起血小板增多和白细胞增多。血小板增多是由血小板生成加速所致，但白细胞增多的机制尚不清楚。本研究旨在确定骨髓刺激和血管内白细胞边缘池解离对IL-6诱导的中性粒细胞增多的相对作用。给家兔静脉注射IL-6（2微克/千克）可引起双相中性粒细胞增多，最初在3小时达到峰值，第二个峰值在9小时出现。使用胸腺嘧啶类似物5'-溴-2'-脱氧尿苷（BrdU）标记骨髓中正在分裂的多形核白细胞（PMN），我们发现IL-6处理在2-6小时将PMN从边缘池动员到循环池中，同时PMN上L-选择素表达降低，并且在12-24小时加速骨髓中PMN从有丝分裂后池的释放。我们得出结论，IL-6引起双相中性粒细胞增多，其中第一个峰值是由于PMN从边缘池动员到循环池中，第二个峰值是由于骨髓中PMN释放加速所致。
