Phillips D L, Xing J, Chong C K, Liu H, Corke H
Departments of Chemistry and Botany, The University of Hong Kong, Pokfulam Road, Hong Kong.
J Agric Food Chem. 2000 Nov;48(11):5105-8. doi: 10.1021/jf9907790.
A method using Raman spectroscopy was recently developed for the determination of the degree of substitution of succinate in waxy maize starch. In this paper it is demonstrated that the method can be generalized to a wide range of starches of different amylose contents and botanical origins. Raman calibration sets were used to form regression equations for five types of succinylated starches, that is, waxy, regular, and two high-amylose maize samples (47 and 66% amylose, respectively) and wheat. The derived calibration curves can be used to find the degree of substitution in samples with unknown levels of succinylation. The Raman calibration lines had linear correlation coefficients of 0.995 or better and enable the fast and nondestructive determination of the degree of substitution of succinate for different types of starches with minimal sample preparation. Also discussed is the potential utility of Raman spectroscopy to simultaneously determine the degree of substitution of succinate and amylose content, using previously determined calibration curves developed for the amylose content of maize starches.
最近开发了一种使用拉曼光谱法测定糯玉米淀粉中琥珀酸酯取代度的方法。本文证明该方法可推广应用于不同直链淀粉含量和植物来源的多种淀粉。使用拉曼校准集为五种类型的琥珀酰化淀粉（即糯玉米、普通玉米以及两种高直链玉米样品，直链淀粉含量分别为47%和66%）和小麦建立回归方程。所得校准曲线可用于测定琥珀酰化水平未知的样品中的取代度。拉曼校准曲线的线性相关系数为0.995或更高，能够在样品制备最少的情况下快速、无损地测定不同类型淀粉中琥珀酸酯的取代度。还讨论了利用先前为玉米淀粉直链淀粉含量建立的校准曲线，拉曼光谱法同时测定琥珀酸酯取代度和直链淀粉含量的潜在用途。
