Suzuki M, Yoshino K, Maeda-Yamamoto M, Miyase T, Sano M
Laboratory of Health Science, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka, Shizuoka 422-8526, Japan.
J Agric Food Chem. 2000 Nov;48(11):5649-53. doi: 10.1021/jf000313d.
The inhibitory effects of tea catechins, the O-methylated derivatives of (-)-epigallocatechin-3-O-gallate (EGCG), and the polyphenol extracts from tea leaves (Camellia sinensis L.) on oxazolone-induced type IV allergy in male ICR mice were investigated. Four major tea catechins and two O-methylated derivatives, (-)-epigallocatechin-3-O-(3-O-methyl)gallate (EGCG3' 'Me) and (-)-epigallocatechin-3-O-(4-O-methyl)gallate (EGCG4' 'Me), showed significant inhibitory effects on mouse type IV allergy after a percutaneous administration at a dose of 0.13 mg/ear. Among tea catechins, the compounds including galloyl moieties, such as EGCG and (-)-epicatechin-3-O-gallate (ECG), showed the strongest inhibitory activities on mouse type IV allergy. The inhibitory activities of EGCG3' 'Me and EGCG4' 'Me were higher than that of EGCG at a dose of 0.05 mg/ear. Polyphenol extract from tea leaves of Benihomare cultivar, which includes EGCG3' 'Me, strongly inhibited mouse type IV allergy after percutaneous administration in comparison with that from Yabukita cultivar, which does not include EGCG3' 'Me, at doses of 0.05 and 0.13 mg/ear. EGCG3' 'Me is thought to contribute, at least in part, to the inhibitory ability of Benihomare tea leaves on mouse type IV allergy. EGCG and the polyphenol extracts from Benihomare and Yabukita tea leaves also inhibited mouse type IV allergy by oral administration at 1 h before the sensitization and at 1 h before the challenge with oxazolone. Therefore, daily intake of tea drinks could have potential to prevent type IV allergy.
研究了茶儿茶素、（-）-表没食子儿茶素-3-O-没食子酸酯（EGCG）的O-甲基化衍生物以及茶叶（茶树）中的多酚提取物对雄性ICR小鼠恶唑酮诱导的IV型过敏的抑制作用。四种主要的茶儿茶素和两种O-甲基化衍生物，即（-）-表没食子儿茶素-3-O-（3-O-甲基）没食子酸酯（EGCG3''Me）和（-）-表没食子儿茶素-3-O-（4-O-甲基）没食子酸酯（EGCG4''Me），在以0.13mg/耳的剂量经皮给药后，对小鼠IV型过敏表现出显著的抑制作用。在茶儿茶素中，含有没食子酰基部分的化合物，如EGCG和（-）-表儿茶素-3-O-没食子酸酯（ECG），对小鼠IV型过敏表现出最强的抑制活性。在0.05mg/耳的剂量下，EGCG3''Me和EGCG4''Me的抑制活性高于EGCG。与不含有EGCG3''Me的薮北品种茶叶相比，含有EGCG3''Me的红映品种茶叶中的多酚提取物在以0.05和0.13mg/耳的剂量经皮给药后，对小鼠IV型过敏有强烈的抑制作用。EGCG3''Me被认为至少部分地促成了红映品种茶叶对小鼠IV型过敏的抑制能力。EGCG以及红映和薮北品种茶叶中的多酚提取物在致敏前1小时和用恶唑酮激发前1小时口服给药时，也能抑制小鼠IV型过敏。因此，日常饮用茶饮料可能具有预防IV型过敏的潜力。
