Ruvoën-Clouet N, Ganière J P, André-Fontaine G, Blanchard D, Le Pendu J
Unité de Pathologie Infectieuse, Ecole Nationale Vétérinaire, 44307 Nantes cedex 03, France.
J Virol. 2000 Dec;74(24):11950-4. doi: 10.1128/jvi.74.24.11950-11954.2000.
The ability of rabbit hemorrhagic disease virus to agglutinate human erythrocytes and to attach to rabbit epithelial cells of the upper respiratory and digestive tracts was shown to depend on the presence of ABH blood group antigens. Indeed, agglutination was inhibited by saliva from secretor individuals but not from nonsecretors, the latter being devoid of H antigen. In addition, erythrocytes of the rare Bombay phenotype, which completely lack ABH antigens, were not agglutinated. Native viral particles from extracts of infected rabbit liver as well as virus-like particles from the recombinant virus capsid protein specifically bound to synthetic A and H type 2 blood group oligosaccharides. Both types of particles could attach to adult rabbit epithelial cells of the upper respiratory and digestive tracts. This binding paralleled that of anti-H type 2 blood group reagents and was inhibited by the H type 2-specific lectin UEA-I and polyacrylamide-conjugated H type 2 trisaccharide. Young rabbit tissues were almost devoid of A and H type 2 antigens, and only very weak binding of virus particles could be obtained on these tissues.
兔出血症病毒凝集人红细胞以及附着于兔上呼吸道和消化道上皮细胞的能力被证明取决于ABH血型抗原的存在。事实上，分泌型个体的唾液可抑制凝集，而非分泌型个体的唾液则不能，后者缺乏H抗原。此外，完全缺乏ABH抗原的罕见孟买血型红细胞不发生凝集。来自感染兔肝脏提取物的天然病毒颗粒以及重组病毒衣壳蛋白形成的病毒样颗粒与合成的A和H2型血型寡糖特异性结合。这两种颗粒均可附着于成年兔上呼吸道和消化道的上皮细胞。这种结合与抗H2型血型试剂的结合相似，并被H2型特异性凝集素UEA-I和聚丙烯酰胺偶联的H2型三糖所抑制。幼兔组织几乎不含A和H2型抗原，在这些组织上只能获得非常微弱的病毒颗粒结合。
