Sánchez D, Tucková L, Sebo P, Michalak M, Whelan A, Sterzl I, Jelínková L, Havrdová E, Imramovská M, Benes Z, Krupicková S, Tlaskalová-Hogenová H
Department of Immunology, Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic.
J Autoimmun. 2000 Dec;15(4):441-9. doi: 10.1006/jaut.2000.0452.
Calreticulin (CRT), a high-affintiy calcium binding protein and chaperone, was recently identified as one of the targets of autoantibodies in coeliac disease. We evaluated the level of IgA and IgG antibodies to CRT in sera from patients with coeliac disease and various autoimmune diseases. The level of antibodies to gliadin (shown previously to cross-react with CTR), isolated enterocytes and tissue transglutaminase were determined for comparison. The mean level of IgA antibodies to CRT was significantly higher (P< 0.001) in sera from coeliac patients with active disease (139.9+/-11.2 AU/+/-SE) than in healthy controls (20.9+/-1.7 AU). In sera of patients with systemic lupus erythematosus (SLE), insulin dependent diabetes mellitus (IDDM), multiple sclerosis (MS) and autoimmune thyroiditis (AT) or inflammatory bowel disease (IBD) the mean level (25.8+/-3.7 to 38.1+/-5.6 AU) did not exceed the cut-off value. A low level of these antibodies, however, was detected in some sera of patients with MS and IBD. The level of IgG anti-CRT antibodies was increased in coeliac patients (mean 125.4+/-8.0 AU, P< 0.001) when compared to that in healthy controls (33.9+/-2.3 AU). The IgG anti-CRT antibodies were also detected in about 30% of SLE patients sera (54.1+/-3.6 AU, P< 0.001), but the mean level reached only half that detected in coeliac patients.
钙网蛋白（CRT）是一种高亲和力钙结合蛋白和伴侣蛋白，最近被确定为乳糜泻自身抗体的靶标之一。我们评估了乳糜泻患者和各种自身免疫性疾病患者血清中抗CRT的IgA和IgG抗体水平。为作比较，还测定了抗麦醇溶蛋白（先前显示与CRT交叉反应）、分离的肠上皮细胞和组织转谷氨酰胺酶的抗体水平。活动期乳糜泻患者血清中抗CRT的IgA抗体平均水平（139.9±11.2 AU/±标准误）显著高于健康对照（20.9±1.7 AU，P<0.001）。在系统性红斑狼疮（SLE）、胰岛素依赖型糖尿病（IDDM）、多发性硬化症（MS）、自身免疫性甲状腺炎（AT）或炎症性肠病（IBD）患者的血清中，平均水平（25.8±3.7至38.1±5.6 AU）未超过临界值。然而，在一些MS和IBD患者的血清中检测到这些抗体水平较低。与健康对照（33.9±2.3 AU）相比，乳糜泻患者中抗CRT的IgG抗体水平升高（平均125.4±8.0 AU，P<0.001）。在约30%的SLE患者血清中也检测到抗CRT的IgG抗体（54.1±3.6 AU，P<0.001），但其平均水平仅为乳糜泻患者检测值的一半。
