Nava F, Carta G
Department of Neuroscience, University of Cagliari, Via Porcell 4, I-09124, Cagliari, Italy.
Int J Immunopharmacol. 2000 Nov;22(11):943-53. doi: 10.1016/s0192-0561(00)00058-8.
In 24 h water and food deprived rats, a single lipopolysaccharide treatment (0.25, 0.50 and 1 mg/kg, i.p.) induced inhibition of thirst and hunger as well as fever. Moreover, the same treatment increased serum cytokines, plasma nitrite/nitrate and corticosterone and urinary prostaglandin levels. In another group of 24 h water and food deprived rats, a repeated lipopolysaccharide treatment (0.25, 0. 50 and 1 mg/kg, i.p.), given at 0, 2, 6, 12 and 24 h, induced tolerance to inhibition of food intake and fever, but not to antidipsogenic effect. Moreover, the same repeated treatment stopped the increase in serum cytokines, plasma corticosterone and urinary prostaglandin concentrations and failed to reduce plasma nitrite/nitrate levels. This data, together with the evidence that a pretreatment with N(G)-nitro-L-arginine methyl ester hydrochloride (L-NAME) (5 and 10 microg per rat) reverses the antidipsogenic effects in lipopolysaccharide tolerant rats, suggests that the persistent reduction of water intake after a repeated lipopolysaccharide treatment is due to the antidipsogenic action of nitric oxide in the brain.
在24小时未饮水和未进食的大鼠中，单次腹腔注射脂多糖（0.25、0.50和1毫克/千克）可抑制口渴和饥饿并引起发热。此外，相同处理可提高血清细胞因子、血浆亚硝酸盐/硝酸盐和皮质酮以及尿液前列腺素水平。在另一组24小时未饮水和未进食的大鼠中，于0、2、6、12和24小时重复腹腔注射脂多糖（0.25、0.50和1毫克/千克）可诱导对食物摄入抑制和发热产生耐受性，但对口渴抑制作用无耐受性。此外，相同的重复处理可阻止血清细胞因子、血浆皮质酮和尿液前列腺素浓度的升高，且未能降低血浆亚硝酸盐/硝酸盐水平。这些数据，连同用盐酸N（G）-硝基-L-精氨酸甲酯（L-NAME）（每只大鼠5和10微克）预处理可逆转脂多糖耐受大鼠的口渴抑制作用这一证据，表明重复注射脂多糖后水摄入量的持续减少是由于大脑中一氧化氮的口渴抑制作用所致。
