D'Hondt K, Heese-Peck A, Riezman H
Biozentrum-University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.
Annu Rev Genet. 2000;34:255-295. doi: 10.1146/annurev.genet.34.1.255.
Genetic and biochemical studies in yeast and animal cells have led to the identification of many components required for endocytosis. In this review, we summarize our understanding of the endocytic machinery with an emphasis on the proteins regulating the internalization step of endocytosis and endosome fusion. Even though the overall endocytic machinery appears to be conserved between yeast and animals, clear differences exist. We also discuss the roles of phosphoinositides, sterols, and sphingolipid precursors in endocytosis, because in addition to proteins, these lipids have emerged as important determinants in the spatial and most likely temporal specificity of endocytic membrane trafficking events.
对酵母和动物细胞进行的遗传学和生物化学研究已鉴定出许多内吞作用所需的组分。在本综述中，我们总结了对胞吞机制的理解，重点关注调节内吞作用内化步骤和内体融合的蛋白质。尽管酵母和动物之间的整体胞吞机制似乎是保守的，但仍存在明显差异。我们还讨论了磷酸肌醇、固醇和鞘脂前体在内吞作用中的作用，因为除蛋白质外，这些脂质已成为内吞膜运输事件空间和很可能时间特异性的重要决定因素。
