Peen E, Williams R C
Department of Medicine, University of Bergen, Haukeland Hospital, Bergen, Norway.
Arthritis Res. 2000;2(4):255-9. doi: 10.1186/ar97. Epub 2000 Jun 12.
Reactive antigenic epitopes on presumed autoantigens of biologic interest have been examined by many researchers. The central third complementarity-determining region (CDR3) residues of a human monoclonal anti-proteinase 3 (PR3) antibody contained many negatively charged aspartic acid residues, perhaps contributing to its reactivity with positively charged PR3 regions. Examination of four other human monoclonal anti-PR3 antibodies shows a number of negatively charged residues within their CDR3 regions. Mapping of segments of linear PR3-epitopes reacting with anti-neutrophil cytoplasmic antibodies (ANCA) demonstrated a preliminary estimate of structures contributing to antigenic determinants. T-cell epitopes on PR3 are reported in studies of chronic myeloid leukemia. These T-cell epitopes appear to be human leukocyte antigen (HLA) A2.1 restricted.
许多研究人员已对具有生物学意义的假定自身抗原上的反应性抗原表位进行了研究。一种人源单克隆抗蛋白酶3（PR3）抗体的中央第三个互补决定区（CDR3）残基包含许多带负电荷的天冬氨酸残基，这可能有助于其与带正电荷的PR3区域发生反应。对另外四种人源单克隆抗PR3抗体的研究表明，它们的CDR3区域内存在一些带负电荷的残基。与抗中性粒细胞胞浆抗体（ANCA）反应的线性PR3表位片段的图谱显示了对有助于抗原决定簇的结构的初步估计。在慢性髓性白血病的研究中报告了PR3上的T细胞表位。这些T细胞表位似乎受人类白细胞抗原（HLA）A2.1限制。
