Sbarbati A, Crescimanno C, Bernardi P, Benati D, Merigo F, Osculati F
Institute of Human Anatomy and Histology, University of Verona, Italy.
Histochem J. 2000 Aug;32(8):483-8. doi: 10.1023/a:1004144317336.
We have studied the postnatal development of the intrinsic nervous system in the circumvallate papilla-vonEbner gland complex using NADPH-diaphorase cytochemistry, immunocytochemistry (for nitric oxide synthase-1 and alpha-internexin) and electron microscopy. In rats sacrificed in their first day post partum (1 p.p.), only isolated NADPH-diaphorase positive neurons were visible in the organ. At 2 p.p., a small group of neurons was visible at the base of the papillae and positive neurons formed short chains close to the developing glandular tubules. In the following days, the NADPH-diapharase positive cells increased in number and nerve fibres were associated to small ganglia located at the base of the papilla or in the gland. After the first week of extrauterine life, the intrinsic nervous system was similar to the intrinsic system of adult animals. An immunocytochemical positivity for nitric oxide synthase-1 appeared at 4 p.p. in neurons located in the gland and at 7 p.p. in cells located at the base of the papilla. Immunocytochemical staining for alpha-internexin showed that at 1 p.p. developing nerve fibres were present in the connective tissue of the tongue's muscle layer. At 2-3 p.p., developing nerve fibres were also present at the bases and in the core of the papilla. In the following days, the positivity for alpha-internexin was reduced and one week after birth was virtually absent. Ultrastructural examination revealed that since 1 p.p. isolated neurons can be found at the base of the papilla. In conclusion, the intrinsic nervous system originates from neurons present in the organ at the birth which, in the first days, undergo a biochemical and morphological maturation while the nerve fibres rapidly grow. These findings support the hypothesis that the intrinsic nervous system of the circumvallate papilla has a role in the maturation of the vonEbner gland.
我们利用NADPH黄递酶细胞化学、免疫细胞化学（针对一氧化氮合酶-1和α-间连蛋白）以及电子显微镜技术，研究了轮廓乳头-味腺复合体中固有神经系统的产后发育情况。在产后第一天（1日龄）处死的大鼠中，该器官内仅可见孤立的NADPH黄递酶阳性神经元。在2日龄时，乳头基部可见一小群神经元，阳性神经元形成靠近发育中的腺管的短链。在接下来的几天里，NADPH黄递酶阳性细胞数量增加，神经纤维与位于乳头基部或腺体内的小神经节相连。宫外生活第一周后，固有神经系统与成年动物的固有系统相似。一氧化氮合酶-1的免疫细胞化学阳性在4日龄时出现在腺体内的神经元中，在7日龄时出现在乳头基部的细胞中。α-间连蛋白的免疫细胞化学染色显示，在1日龄时，发育中的神经纤维存在于舌肌层的结缔组织中。在2 - 3日龄时，乳头基部和核心也有发育中的神经纤维。在接下来的几天里，α-间连蛋白的阳性反应减少，出生一周后几乎消失。超微结构检查显示，自1日龄起，乳头基部可发现孤立的神经元。总之，固有神经系统起源于出生时器官内存在的神经元，在最初几天，这些神经元经历生化和形态成熟，而神经纤维迅速生长。这些发现支持了轮廓乳头固有神经系统在味腺成熟中起作用的假说。
