Afework M, Tomlinson A, Burnstock G
Department of Anatomy and Developmental Biology, University College London, UK.
Cell Tissue Res. 1994 Apr;276(1):133-41. doi: 10.1007/BF00354792.
The distribution and colocalization of nitric oxide synthase and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-diaphorase) was investigated in the adrenal gland of developing, adult and aging rats with the use of immunohistochemical and histochemical techniques. Nitric oxide synthase-immunoreactive neurons within the adrenal gland were found from the 20th day of gestation onwards. During early development the neurons were found as small clusters of smaller-size cells compared to those observed in the adult gland. Their number reached that of adult level by the 4th day after birth, and in the glands from aging rats a 28.6% increase was observed. Whilst no immunofluorescence was seen in chromaffin cells during early development, some cells from glands of aging rats showed nitric oxide synthase-immunoreactivity with varying intensity. The immunoreactive neurons from postnatal rat adrenals were also positive for NADPH-diaphorase, whilst those in prenatal rats were negative or lightly stained. Nitric oxide synthase-immunoreactive nerve fibres were present in all adrenal glands examined from the 16th day of gestation onwards. A considerable degree of variation in the distribution of immunoreactive fibres both in medulla and outer region of cortex at the different age groups was observed and described. Most, but not all, nitric oxide synthase-immunoreactive nerve fibres also showed NADPH-diaphorase staining.
采用免疫组织化学和组织化学技术，研究了发育中、成年和老龄大鼠肾上腺中一氧化氮合酶和烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH - 黄递酶）的分布及共定位情况。从妊娠第20天起，在肾上腺中发现了一氧化氮合酶免疫反应性神经元。在发育早期，与成年腺体中观察到的神经元相比，这些神经元是较小尺寸细胞的小簇。它们的数量在出生后第4天达到成年水平，并且在老龄大鼠的腺体中观察到增加了28.6%。在发育早期，嗜铬细胞中未见免疫荧光，而老龄大鼠腺体中的一些细胞显示出不同强度的一氧化氮合酶免疫反应性。出生后大鼠肾上腺中的免疫反应性神经元对NADPH - 黄递酶也呈阳性，而产前大鼠中的神经元呈阴性或轻度染色。从妊娠第16天起，在所检查的所有肾上腺中均存在一氧化氮合酶免疫反应性神经纤维。观察并描述了不同年龄组髓质和皮质外层区域免疫反应性纤维分布的相当大程度的变化。大多数但并非所有的一氧化氮合酶免疫反应性神经纤维也显示出NADPH - 黄递酶染色。
