Nogami M, Takatsu A, Endo N, Ishiyama I
Department of Legal Medicine, Teikyo University School of Medicine, Tokyo, Japan.
Histochem J. 2000 Aug;32(8):505-8. doi: 10.1023/a:1004108804132.
Cathepsin D is a lysosomal enzyme involved in neuronal degeneration. In this study, the immunohistochemistry of cathepsin D was studied in hippocampal CA1 neurons that are vulnerable to ischemia, and parahippocampal glial cells. CA1 neurons from the majority of cases showed cathepsin D immunoreactivity in the cytoplasm, whereas shrunk neurons were unstained in only one case. There was no statistically significant correlation between the postmortem interval between death and autopsy, and cathepsin D immunoreactivity in CA1 neurons. These observations indicate that cathepsin D immunoreactivity is not a sensitive marker for neuronal degeneration or postmortem changes. On the other hand, there was a statistically significant correlation between age and cathepsin D immunoreactivity in the cytoplasm of parahippocampal glial cells. This shows that senescence is correlated with cathepsin D expression in humans as has been reported previously in an animal study.
组织蛋白酶D是一种参与神经元变性的溶酶体酶。在本研究中，对易受缺血影响的海马CA1神经元和海马旁神经胶质细胞进行了组织蛋白酶D的免疫组织化学研究。大多数病例的CA1神经元在细胞质中显示组织蛋白酶D免疫反应性，而仅在1例中萎缩的神经元未染色。死亡与尸检之间的死后间隔与CA1神经元中的组织蛋白酶D免疫反应性之间无统计学显著相关性。这些观察结果表明，组织蛋白酶D免疫反应性不是神经元变性或死后变化的敏感标志物。另一方面，海马旁神经胶质细胞质中的年龄与组织蛋白酶D免疫反应性之间存在统计学显著相关性。这表明衰老与组织蛋白酶D表达相关，正如先前在一项动物研究中所报道的那样。
