Visioli F, Caruso D, Galli C, Viappiani S, Galli G, Sala A
Institute of Pharmacological Sciences, Milan, Italy.
Biochem Biophys Res Commun. 2000 Nov 30;278(3):797-9. doi: 10.1006/bbrc.2000.3879.
This study was undertaken to evaluate, in humans, the antioxidant activity of olive oil phenolics, namely hydroxytyrosol and oleuropein aglycone that share an orthodiphenolic (catecholic) structure. Human volunteers were administered olive oil samples containing increasing amounts of an olive oil phenolic extract that was characterized by gas-chromatography/mass spectrometry. The administration of phenol-rich oils was dose-dependently associated with a decreased urinary excretion of 8-iso-PGF(2alpha), a biomarker of oxidative stress. Also, a statistically significant negative correlation between homovanillyl alcohol (HValc, hydroxytyrosol's major metabolite, formed through the COMT system) and F(2)-isoprostanes excretion was found. Thus, the administration of oil samples with increasing, albeit low, concentrations of orthodiphenolic compounds, namely hydroxytyrosol and oleuropein aglycone, results in a dose-dependent reduction in the urinary excretion of 8-iso-PGF(2alpha). The statistically significant negative correlation between 8-iso-PGF(2alpha) and HValc urinary concentrations suggests that this metabolite better reflects the in vivo activities of hydroxytyrosol.
本研究旨在评估橄榄油酚类物质（即具有邻二酚（儿茶酚）结构的羟基酪醇和橄榄苦苷配基）在人体中的抗氧化活性。给人类志愿者服用含有通过气相色谱/质谱法表征的、含量不断增加的橄榄油酚类提取物的橄榄油样品。富含酚类的油的服用与氧化应激生物标志物8-异前列腺素F2α（8-iso-PGF(2α)）的尿排泄减少呈剂量依赖性相关。此外，还发现高香草醇（HValc，羟基酪醇通过儿茶酚-O-甲基转移酶（COMT）系统形成的主要代谢产物）与F2-异前列腺素排泄之间存在统计学上显著的负相关。因此，服用含有浓度不断增加（尽管较低）的邻二酚化合物（即羟基酪醇和橄榄苦苷配基）的油样，会导致8-iso-PGF(2α)的尿排泄呈剂量依赖性减少。8-iso-PGF(2α)与HValc尿浓度之间统计学上显著的负相关表明，这种代谢产物能更好地反映羟基酪醇的体内活性。
