Visioli F, Galli C, Plasmati E, Viappiani S, Hernandez A, Colombo C, Sala A
University of Milan, Institute of Pharmacological Sciences, Milan, Italy.
Circulation. 2000 Oct 31;102(18):2169-71. doi: 10.1161/01.cir.102.18.2169.
Oxidative stress is involved in the onset of several degenerative disorders, and epidemiological studies indicate that a high intake of dietary antioxidants, as in the case of the Mediterranean basin, is protective. Olive mill waste waters (OMWWs) are a byproduct of olive oil production rich in phenolic antioxidants, such as hydroxytyrosol. We tested the effects of a low dose of an OMWW extract in a model of sidestream smoke-induced oxidative stress in rats by evaluating the urinary excretion of 8-iso-prostaglandin (PG) F(2alpha) (iPF(2alpha)-III).
An OMWW extract (5 mg/kg, providing 414 microg/kg of hydroxytyrosol) was administered to rats daily for 4 days, during which time the animals were exposed to sidestream smoke for 20 minutes once a day. Daily urines were collected, and the urinary excretion of 8-iso-PGF(2alpha) was evaluated as an index of oxidative stress-induced in vivo lipid peroxidation. The exposure of rats to passive smoking increased the urinary excretion of 8-iso-PGF(2alpha) by 44+/-4.2% at 48 hours and by 55+/-10% at 96 hours. Treatment with the OMWW extract was able to completely prevent the increase at 48 hours and resulted in lower increments (34+/-18% versus 55+/-10%) of 8-iso-PGF(2alpha) excretion at 96 hours.
A low dose of hydroxytyrosol, administered through OMWW, reduces the consequences of sidestream smoke-induced oxidative stress in rats.
氧化应激参与多种退行性疾病的发病过程，流行病学研究表明，如地中海地区那样高摄入膳食抗氧化剂具有保护作用。橄榄油厂废水（OMWWs）是橄榄油生产的副产物，富含酚类抗氧化剂，如羟基酪醇。我们通过评估8-异前列腺素（PG）F2α（iPF2α-III）的尿排泄量，测试了低剂量OMWW提取物在大鼠侧流烟雾诱导的氧化应激模型中的作用。
将OMWW提取物（5mg/kg，提供414μg/kg羟基酪醇）每日给予大鼠，持续4天，在此期间动物每天暴露于侧流烟雾20分钟。每天收集尿液，并评估8-异前列腺素F2α的尿排泄量，作为体内氧化应激诱导的脂质过氧化的指标。大鼠被动吸烟暴露在48小时时使8-异前列腺素F2α的尿排泄量增加44±4.2%，在96小时时增加55±10%。用OMWW提取物治疗能够完全阻止48小时时的增加，并导致96小时时8-异前列腺素F2α排泄量的增加幅度降低（34±18%对55±10%）。
通过OMWW给予低剂量的羟基酪醇可减轻大鼠侧流烟雾诱导的氧化应激后果。
