DeSombre E R, Anderson W A, Kang Y H
Cancer Res. 1975 Jan;35(1):172-9.
An estrogen-induced, intensely staining peroxidase 3,3-diaminobenzidine-positive reaction product is found to be characteristic of hormone-dependent, 7,12-dimenthylbenz(a)anthracene-induced mammary tumors of the rat. This product is demonstrated in thick sections of such tumors from intact or estrogen-treated castrate rats but is not seen in tumors that are in regression due to castration or estrogen deprivation. It is, furthermore, absent from tumors whose growth is unaffected by castration. The subcellular localization of this enzyme activity is restricted mainly to the nuclear envelope and cisternae of the granular endoplasmic reticulum in addition to secretory granules. This provides the first evidence for a criterion that would allow differentiation of hormone-dependent and hormone-independent mammary cancer on histological sections and, as such, may have considerable potential as an aid in the classification of human breast cancer.
雌激素诱导的、染色强烈的过氧化物酶3,3 - 二氨基联苯胺阳性反应产物被发现是激素依赖性、7,12 - 二甲基苯并(a)蒽诱导的大鼠乳腺肿瘤的特征。在完整或经雌激素处理的去势大鼠的此类肿瘤厚切片中可显示该产物，但在因去势或雌激素剥夺而消退的肿瘤中未见。此外，其生长不受去势影响的肿瘤中也不存在该产物。这种酶活性的亚细胞定位主要局限于核膜、颗粒内质网的池以及分泌颗粒。这为在组织学切片上区分激素依赖性和激素非依赖性乳腺癌的标准提供了首个证据，因此，在人类乳腺癌分类辅助方面可能具有相当大的潜力。
