Penney G C, Hawkins R A
Histochem J. 1981 Nov;13(6):983-8. doi: 10.1007/BF01002638.
The endogenous peroxidase content of 26 'hormone-dependent' and 16 'hormone-independent' rat mammary tumours was assessed by means of a biochemical (guaiacol) assay on tumour extracts and by means of a histochemical (diaminobenzidine) technique on frozen sections of the same tumours. By guaiacol assay, the hormone-dependent tumours had significantly higher peroxidase levels than the hormone-independent tumours. In contrast, by diaminobenzidine staining of the same tumours, peroxidase was detectable in 94% of hormone-independent tumours but in only 54% of hormone-dependent tumours. Moreover, there was no direct correlation between the results of biochemical and histochemical methods. At least in these rat mammary tumours, therefore, histochemical estimates of peroxidase activity based on the diaminobenzidine reaction do not seem to reflect the same tissue properties as biochemical estimates based on the guaiacol reaction after tissue disruption.
通过对肿瘤提取物进行生化（愈创木酚）测定以及对相同肿瘤的冰冻切片进行组织化学（二氨基联苯胺）技术，评估了26个“激素依赖性”和16个“激素非依赖性”大鼠乳腺肿瘤的内源性过氧化物酶含量。通过愈创木酚测定，激素依赖性肿瘤的过氧化物酶水平显著高于激素非依赖性肿瘤。相比之下，对相同肿瘤进行二氨基联苯胺染色时，94%的激素非依赖性肿瘤中可检测到过氧化物酶，而激素依赖性肿瘤中仅为54%。此外，生化方法和组织化学方法的结果之间没有直接相关性。因此，至少在这些大鼠乳腺肿瘤中，基于二氨基联苯胺反应的过氧化物酶活性的组织化学估计似乎并不能反映与基于组织破坏后愈创木酚反应的生化估计相同的组织特性。
