Yokomaku D, Yamaguchi N, Nasu M
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka 565-0871, Japan.
Appl Environ Microbiol. 2000 Dec;66(12):5544-8. doi: 10.1128/AEM.66.12.5544-5548.2000.
A direct viable count (DVC) procedure was developed which clearly and easily discriminates the viability of bacterial cells. In this quantitative DVC (qDVC) procedure, viable cells are selectively lysed by spheroplast formation caused by incubation with antibiotics and glycine. This glycine effect leads to swollen cells with a very loose cell wall. The viable cells then are lysed easily by a single freeze-thaw treatment. The number of viable cells was obtained by subtracting the number of remaining cells after the qDVC procedure from the total cell number before the qDVC incubation. This improved procedure should provide useful information about the metabolic potential of natural bacterial communities.
开发了一种直接活菌计数（DVC）方法，该方法能够清晰、简便地鉴别细菌细胞的活力。在这种定量DVC（qDVC）方法中，活细胞通过与抗生素和甘氨酸孵育导致原生质球形成而被选择性裂解。这种甘氨酸效应导致细胞肿胀，细胞壁非常疏松。然后通过单次冻融处理即可轻松裂解活细胞。活细胞数量通过从qDVC孵育前的总细胞数中减去qDVC程序后剩余的细胞数来获得。这种改进的方法应该能够提供有关天然细菌群落代谢潜力的有用信息。
