Western K H, Videm V
Department of Immunology, Institute of Laboratory Medicine, the Regional Hospital, Trondheim, Norway.
Transfusion. 2000 Nov;40(11):1414-8. doi: 10.1046/j.1537-2995.2000.40111414.x.
Neutrophils are important mediators of inflammation and may be activated by foreign surfaces in apheresis systems. Because most of the WBCs are returned to the donor, it was investigated whether artificial activation leads to altered donor neutrophil function.
Three apheresis systems (Amicus, Autopheresis-C, and CS-3000; all: Baxter Fenwal) were investigated. Preapheresis and postapheresis blood samples were drawn from 10 volunteer donors, with all three apheresis systems used in random order for each donor. Changes in neutrophil phagocytic ability, oxidative burst, and expression of L-selectin and CD11b were measured by flow cytometry, and plasma concentrations of myeloperoxidase and lactoferrin were measured by EIA. Complement activation was evaluated by quantification of C3bc and the terminal complement complex by EIA.
Neutrophil expression of L-selectin increased after apheresis (p = 0.02), and the production of oxygen radicals was reduced (p = 0.01). This effect was possibly a result of priming. Complement was not activated. There were no significant differences in neutrophil function after apheresis with any of the three apheresis systems.
Neutrophil function was altered after apheresis, although to a very small extent, and contact between neutrophils and the foreign surface in the apheresis systems is found to be a biotolerant procedure.
中性粒细胞是炎症的重要介质，可能会被血液成分单采系统中的外来表面激活。由于大多数白细胞会回输给献血者，因此研究了人工激活是否会导致献血者中性粒细胞功能改变。
研究了三种血液成分单采系统（阿密库斯血细胞分离机、自采血细胞分离机-C和CS-3000；均为百特芬华公司产品）。从10名志愿献血者采集单采前和单采后的血样，对每位献血者随机依次使用所有三种血液成分单采系统。通过流式细胞术检测中性粒细胞吞噬能力、氧化爆发以及L-选择素和CD11b的表达变化，通过酶免疫测定法检测血浆髓过氧化物酶和乳铁蛋白浓度。通过酶免疫测定法定量C3bc和末端补体复合物来评估补体激活情况。
单采后中性粒细胞L-选择素表达增加（p = 0.02），氧自由基产生减少（p = 0.01）。这种效应可能是预激的结果。补体未被激活。使用三种血液成分单采系统中的任何一种进行单采后，中性粒细胞功能均无显著差异。
单采后中性粒细胞功能发生改变，尽管程度非常小，并且发现中性粒细胞与血液成分单采系统中的外来表面接触是一种生物耐受性操作。
