新型隐球菌对四种抗真菌药物的抗真菌药敏试验中Etest法与微量稀释法的比较。

Comparison of the Etest and microdilution method for antifungal susceptibility testing of Cryptococcus neoformans to four antifungal agents.

作者信息

Aller A I, Martín-Mazuelos E, Gutiérrez M J, Bernal S, Chávez M, Recio F J

机构信息

Servicio de Microbiología, Hospital Universitario de Valme, Carretera de Cádiz s/n, E-41014 Seville, Spain.

出版信息

J Antimicrob Chemother. 2000 Dec;46(6):997-1000. doi: 10.1093/jac/46.6.997.

DOI:10.1093/jac/46.6.997

PMID:11102421

Abstract

We performed a prospective study to compare the Etest and the microdilution method (NCCLS guidelines) for determining the MICs of fluconazole, itraconazole, flucytosine and amphotericin B for 35 strains of Cryptococcus neoformans. For the microdilution method (MDM) RPMI 1640 medium with 2% glucose was used for fluconazole, itraconazole and flucytosine, and Antibiotic Medium 3 for amphotericin B. For the Etest, RPMI 1640 medium with 2% glucose and solidified with 1.5% agar was used for the four antifungal agents. Amphotericin B was also tested on Antibiotic Medium 3 solidified with 1.5% agar. Fluconazole and flucytosine MICs by the Etest showed good correlation with the broth MDM (81.1 and 89.2% agreement within two dilutions, respectively). With the tested population of itraconazole- and amphotericin B-susceptible isolates, the MIC agreement for itraconazole was 54%; amphotericin B showed the lowest agreement (8.1% on Antibiotic Medium 3 and 13.5% on RPMI).

摘要

我们进行了一项前瞻性研究，以比较Etest法和微量稀释法（NCCLS指南）对35株新型隐球菌测定氟康唑、伊曲康唑、氟胞嘧啶和两性霉素B的最低抑菌浓度（MIC）。对于微量稀释法（MDM），含2%葡萄糖的RPMI 1640培养基用于氟康唑、伊曲康唑和氟胞嘧啶，含两性霉素B的培养基3用于两性霉素B。对于Etest法，含2%葡萄糖并添加1.5%琼脂固化的RPMI 1640培养基用于这四种抗真菌剂。两性霉素B也在添加1.5%琼脂固化的培养基3上进行测试。通过Etest法测定的氟康唑和氟胞嘧啶的MIC与肉汤微量稀释法显示出良好的相关性（分别在两个稀释度内一致性为81.1%和89.2%）。对于测试的伊曲康唑和两性霉素B敏感分离株群体，伊曲康唑的MIC一致性为54%；两性霉素B显示出最低的一致性（在培养基3上为8.1%，在RPMI上为13.5%）。