新型隐球菌药敏试验中 MIC 测试条和 Sensititre YeastOne 与 CLSI 和 EUCAST 肉汤微量稀释参考方法的比较。
Comparison of MIC Test Strip and Sensititre YeastOne with the CLSI and EUCAST Broth Microdilution Reference Methods for Antifungal Susceptibility Testing of Cryptococcus neoformans.
机构信息
Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands.
Foundation Atlas of Clinical Fungi, Hilversum, The Netherlands.
出版信息
Antimicrob Agents Chemother. 2020 Mar 24;64(4). doi: 10.1128/AAC.02261-19.
Abstract
We compared MIC test strip (MTS) and Sensititre YeastOne (SYO) methods with EUCAST and CLSI methods for amphotericin B, 5-fluocytosine, fluconazole, voriconazole, and isavuconazole against 106 isolates. The overall essential agreement between the EUCAST and CLSI methods was >72% and >94% at ±1 and ±2 dilutions, respectively. The essential agreements between SYO and EUCAST/CLSI for amphotericin B, 5-flucytosine, fluconazole, and voriconazole were >89/>93% and between MTS and EUCAST/CLSI were >57/>75%. Very major error rates were low for amphotericin B and fluconazole (<3%) and a bit higher for the other drugs (<8%).
摘要
我们比较了 MIC 测试条（MTS）和 Sensititre YeastOne（SYO）方法与 EUCAST 和 CLSI 方法在抗真菌药物两性霉素 B、5-氟胞嘧啶、氟康唑、伏立康唑和伊曲康唑对 106 株分离株的效果。EUCAST 和 CLSI 方法之间的总体基本一致率分别为±1 和±2 稀释度时均>72%和>94%。SYO 与 EUCAST/CLSI 之间的基本一致率对两性霉素 B、5-氟胞嘧啶、氟康唑和伏立康唑分别为>89%/>93%，MTS 与 EUCAST/CLSI 之间分别为>57%/>75%。两性霉素 B 和氟康唑的非常大误差率较低（<3%），其他药物的误差率略高（<8%）。
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