Del Poeta M, Barchiesi F, Morbiducci V, Arzeni D, Marinucci G, Ancarani F, Scalise G
Institute of Infectious Diseases and Public Health, University of Ancona, Ospedale Umberto I., Italy.
J Chemother. 1994 Jun;6(3):173-6. doi: 10.1080/1120009x.1994.11741148.
We compared the in vitro activity of amphotericin B, flucytosine, itraconazole, fluconazole, ketoconazole and miconazole against 18 strains of Cryptococcus neoformans by using two methods: microbroth dilution and semisolid agar dilution. By both of the methods minimum inhibitory concentrations (MICs) showed a wide range for all antifungal agents but not for amphotericin B. Statistically significant differences between the two methods were observed only with amphotericin B and flucytosine, p = 0.048 and p = 0.045 respectively. Our study suggests that azole susceptibility testing for C. neoformans may be performed by the broth microdilution as well as the semisolid agar test. The choice of the method when testing amphotericin B and flucytosine is more problematic.
我们采用微量肉汤稀释法和半固体琼脂稀释法，比较了两性霉素B、氟胞嘧啶、伊曲康唑、氟康唑、酮康唑和咪康唑对18株新型隐球菌的体外活性。通过这两种方法，所有抗真菌药物的最低抑菌浓度（MIC）均显示出较宽的范围，但两性霉素B除外。仅在两性霉素B和氟胞嘧啶中观察到两种方法之间具有统计学意义的差异，p值分别为0.048和0.045。我们的研究表明，新型隐球菌的唑类药敏试验可通过肉汤微量稀释法以及半固体琼脂试验进行。在检测两性霉素B和氟胞嘧啶时，方法的选择更具问题。
