Kelch A, Wessel S, Will T, Hintze U, Wepf R, Wiesendanger R
Analytical Research, Beiersdorf AG, Department of Analytical Research, Troplowitzstr. 15, 22529 Hamburg, Germany.
J Microsc. 2000 Dec;200(Pt 3):179-86. doi: 10.1046/j.1365-2818.2000.00756.x.
Thin cross-sections of human hairs were investigated by scanning near-field optical microscopy (SNOM) and confocal laser scanning microscopy (CLSM) after penetration of a fluorescent dye. The same samples were measured with both techniques to compare the observed structures. The images obtained from the two methods show nearly identical structures representing pathways of the dye molecules in hairs. The SNOM images provide a higher resolution than the CLSM images. Therefore, SNOM is believed to be a suitable method for investigations at a resolution of 100 nm on penetration pathways of fluorescent dyes such as the cell membrane complex pathway in cross-sections of hairs.
在荧光染料渗透后，通过扫描近场光学显微镜（SNOM）和共聚焦激光扫描显微镜（CLSM）对人发的薄切片进行了研究。用这两种技术对相同的样品进行测量，以比较观察到的结构。从这两种方法获得的图像显示出几乎相同的结构，代表了毛发中染料分子的路径。SNOM图像比CLSM图像具有更高的分辨率。因此，SNOM被认为是一种适用于以100 nm分辨率研究荧光染料渗透路径的方法，例如毛发横截面中的细胞膜复合路径。
