Meixner A J, Kneppe H
Universität-Gesamthochschule Siegen, Germany.
Cell Mol Biol (Noisy-le-grand). 1998 Jul;44(5):673-88.
Scanning a point light source in close proximity over a sample and recording the scattered or transmitted light intensity point by point allows one to record optical images with a resolution not limited by diffraction. An overview of this technique called scanning near-field optical microscopy (SNOM or NSOM) is given with emphasis on cell- and microbiology. After an introduction, where the basic features of the technique are explained, illustrative examples are presented, such as a HeLa cell, fluorescence labelled human chromosomes, super resolution fluorescence imaging, single molecule imaging and fluorescence resonance energy transfer between a single pair of dye molecules.
在样品上方近距离扫描点光源并逐点记录散射光或透射光强度，能够记录分辨率不受衍射限制的光学图像。本文概述了一种名为扫描近场光学显微镜（SNOM或NSOM）的技术，重点介绍了其在细胞和微生物学方面的应用。在引言部分解释了该技术的基本特征后，给出了一些示例，如HeLa细胞、荧光标记的人类染色体、超分辨率荧光成像、单分子成像以及单对染料分子之间的荧光共振能量转移。
