Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand.
Int J Pharm. 2013 Jan 30;441(1-2):151-61. doi: 10.1016/j.ijpharm.2012.12.003. Epub 2012 Dec 13.
The objective of this study was to elucidate the skin penetration pathway of the generated ultradeformable liposomes (ULs) with terpenes for transdermal drug delivery of fluorescein sodium (NaFl). ULs with d-limonene were selected to investigate the penetration pathways and vesicle-skin interaction in terms of release and attachment processes via Confocal Laser Scanning Microscopy (CLSM). A co-localization technique was employed to visualize the skin penetration behavior of UL-labeled red fluorescence (Rh-PE) and fluorescence-entrapped drug (NaFl) through porcine skin. Our results suggested that ULs with entrapped drug might attach to any part of the skin before releasing the entrapped drug into the skin. Most ULs and entrapped drug penetrated through hair follicles more than through the nonfollicular region. In summary, the transfollicular pathway was the major penetration pathway of ULs with d-limonene for transdermal drug delivery of NaFl, whereas the intercellular and transcellular pathways were the minor penetration pathways.
本研究旨在阐明生成的具有萜类化合物的超变形脂质体 (UL) 的皮肤渗透途径，用于荧光素钠 (NaFl) 的透皮药物传递。选择含有 d-柠檬烯的 UL 来研究渗透途径和囊泡 - 皮肤相互作用，包括释放和附着过程，通过共聚焦激光扫描显微镜 (CLSM)。采用共定位技术可视化通过猪皮的 UL 标记红色荧光 (Rh-PE) 和荧光包封药物 (NaFl) 的皮肤渗透行为。我们的结果表明，包封药物的 UL 可能在将包封药物释放到皮肤中之前附着在皮肤的任何部位。大多数 UL 和包封药物通过毛囊渗透的程度超过非毛囊区域。总之，对于 d-柠檬烯包封的 UL 透皮传递 NaFl，穿细胞途径是主要的渗透途径，而细胞间和跨细胞途径是次要的渗透途径。
