Golovko A, Hjälm G, Sitbon F, Nicander B
Department of Plant Biology, SLU, PO Box 7080, SE-75007, Uppsala, Sweden.
Gene. 2000 Nov 27;258(1-2):85-93. doi: 10.1016/s0378-1119(00)00421-2.
A cDNA of human origin is shown to encode a tRNA isopentenyl transferase (E.C. 2.5.1.8). Expression of the gene in a Saccharomyces cerevisiae mutant lacking the endogenous tRNA isopentenyl transferase MOD5 resulted in functional complementation and reintroduction of isopentenyladenosine into tRNA. The deduced amino acid sequence contains a number of regions conserved in known tRNA isopentenyl transferases. The similarity to the S. cerevisiae MOD5 protein is 53%, and to the Escherichia coli MiaA protein 47%. The human sequence was found to contain a single C2H2 Zn-finger-like motif, which was detected also in the MOD5 protein, and several putative tRNA transferases located by BLAST searches, but not in prokaryotic homologues.
一种源自人类的cDNA被证明可编码一种tRNA异戊烯基转移酶（酶编号：2.5.1.8）。该基因在缺乏内源性tRNA异戊烯基转移酶MOD5的酿酒酵母突变体中的表达导致功能互补，并使异戊烯基腺苷重新引入tRNA。推导的氨基酸序列包含许多在已知tRNA异戊烯基转移酶中保守的区域。与酿酒酵母MOD5蛋白的相似性为53%，与大肠杆菌MiaA蛋白的相似性为47%。发现人类序列包含一个单一的C2H2锌指样基序，该基序在MOD5蛋白中也被检测到，并且通过BLAST搜索定位到了几个推定的tRNA转移酶，但在原核同源物中未发现。
