Delourme D, Lacroute F, Karst F
Laboratoire de Génétique Physiologique et Moléculaire, Université de Poitiers, France.
Plant Mol Biol. 1994 Dec;26(6):1867-73. doi: 10.1007/BF00019499.
A cDNA encoding farnesyl diphosphate synthase, an enzyme that synthesizes C15 isoprenoid diphosphate from isopentenyl diphosphate and dimethylallyl diphosphate, was cloned from an Arabidopsis thaliana cDNA library by complementation of a mutant of Saccharomyces cerevisiae deficient in this enzyme. The A. thaliana cDNA was also able to complement the lethal phenotype of the erg20 deletion yeast mutant. As deduced from the full-length 1.22 kb cDNA nucleotide sequence, the polypeptide contains 343 amino acids and has a relative molecular mass of 39,689. The predicted amino acid sequence presents about 50% identity with the yeast, rat and human FPP synthases. Southern blot analyses indicate that A. thaliana probably contains a single gene for farnesyl diphosphate synthase.
从拟南芥cDNA文库中通过对缺乏法尼基二磷酸合酶的酿酒酵母突变体进行互补克隆得到了一个编码法尼基二磷酸合酶的cDNA，该酶可由异戊烯基二磷酸和二甲基烯丙基二磷酸合成C15类异戊二烯二磷酸。拟南芥cDNA也能够互补erg20缺失酵母突变体的致死表型。根据全长1.22 kb cDNA核苷酸序列推导，该多肽含有343个氨基酸，相对分子质量为39,689。预测的氨基酸序列与酵母、大鼠和人类的FPP合酶具有约50%的同一性。Southern杂交分析表明，拟南芥可能含有单个法尼基二磷酸合酶基因。
