Hirano Y, Tamura M, Hayashi K
Department of Bacteriology, Nihon University School of Dentistry, Tokyo, Japan.
J Oral Sci. 2000 Sep;42(3):125-31. doi: 10.2334/josnusd.42.125.
Prevotella nigrescens ATCC 25261 (P. nigrescens) cells adhere well to hydroxyapatite treated with citrate (CHA), but the attachment is drastically inhibited by lactoferrin (LF). To determine the nature of the iron-free LF responsible for inhibiting P. nigrescens cell attachment, this study tested the duration and frequency of LF treatment of CHA and the effects of divalent and trivalent ferric ions. The inhibitory effect on the attachment of P. nigrescens was somewhat higher with bovine LF than human LF. Apo LF effectively inhibited P. nigrescens cell attachment to CHA, and almost abolished attachment at a concentration of 0.4 mg/ml. Fe3+ saturated LF was unable to inhibit attachment, whereas Fe2+ showed a slight effect under the same conditions. The LF absorbed rapidly to CHA in less than 10 min. With a lower concentration (0.1 mg/ml) of LF, only three treatments of CHA were required for the maximum inhibition of P. nigrescens cell attachment. The quantity of LF adsorbed to the hydroxyapatite (HA) and to P. nigrescens cells was determined by use of [3H]-LF. Approximately 25 micrograms of LF protein adsorbed to 5 mg of HA at saturation, and approximately 0.25 microgram of LF did so to the 6 x 10(8) cells.
变黑普雷沃菌ATCC 25261（变黑普雷沃菌）细胞能很好地黏附于经柠檬酸盐处理的羟基磷灰石（CHA），但乳铁蛋白（LF）会显著抑制这种黏附。为确定负责抑制变黑普雷沃菌细胞黏附的无铁LF的性质，本研究测试了LF处理CHA的持续时间和频率以及二价和三价铁离子的影响。牛LF对变黑普雷沃菌黏附的抑制作用略高于人LF。脱铁乳铁蛋白能有效抑制变黑普雷沃菌细胞黏附于CHA，在浓度为0.4mg/ml时几乎完全消除黏附。Fe3+饱和的LF无法抑制黏附，而Fe2+在相同条件下有轻微作用。LF在不到10分钟内迅速吸附到CHA上。使用较低浓度（0.1mg/ml）的LF时，对CHA进行三次处理就能最大程度抑制变黑普雷沃菌细胞黏附。通过使用[3H]-LF测定吸附到羟基磷灰石（HA）和变黑普雷沃菌细胞上的LF量。饱和时，约25微克LF蛋白吸附到5毫克HA上，约0.25微克LF吸附到6×108个细胞上。
