Alugupalli K R, Kalfas S
Department of Oral Microbiology, University Hospital MAS, Lund University, Malmö, Sweden.
APMIS. 1997 Sep;105(9):680-8. doi: 10.1111/j.1699-0463.1997.tb05071.x.
Lactoferrin was previously shown to inhibit the adhesion of A. actinomycetemcomitans, P. intermedia and P. nigrescens to human cells. Lactoferrin was also shown to competitively inhibit the binding of these bacteria to the basement membrane protein laminin. The present study aimed to determine the type of interactions inhibited by lactoferrin. Lactoferrin binds to fibroblast monolayers and Matrigel, a reconstituted basement membrane, through ionic interactions. The adhesion of A. actinomycetemcomitans to these substrata was mainly dependent on the ionic strength of the environment. P. intermedia and P. nigrescens also adhere to fibroblasts mainly by ionic interactions, while their adhesion to Matrigel seems to be mediated by specific mechanisms. Lectin-type interactions were not found to be involved in the binding of these bacteria to the substrata. Treatment of either A. actinomycetemcomitans or fibroblasts with lactoferrin decreased the adhesion in a dose-dependent manner, while lactoferrin treatment of Matrigel alone had no adhesion-counteracting effect. Adhesion of P. intermedia and P. nigrescens to Matrigel was not significantly affected by the ionic strength, but the presence of lactoferrin inhibited the adhesion. Lactoferrin bound to Matrigel, P. intermedia and P. nigrescens was rapidly released, while lactoferrin bound to A. actinomycetemcomitans and fibroblasts was retained. These findings indicate that lactoferrin-dependent inhibition of the adhesion of A. actinomycetemcomitans, P. intermedia and P. nigrescens to fibroblasts and Matrigel can involve binding of lactoferrin to both the bacteria and substrata. The decreased adhesion may be due to blocking of both specific adhesin-ligand as well as non-specific charge-dependent interactions.
乳铁蛋白先前已被证明可抑制伴放线放线杆菌、中间普氏菌和变黑普氏菌对人细胞的黏附。乳铁蛋白还被证明可竞争性抑制这些细菌与基底膜蛋白层粘连蛋白的结合。本研究旨在确定乳铁蛋白所抑制的相互作用类型。乳铁蛋白通过离子相互作用与成纤维细胞单层和基质胶（一种重组基底膜）结合。伴放线放线杆菌对这些底物的黏附主要取决于环境的离子强度。中间普氏菌和变黑普氏菌也主要通过离子相互作用黏附于成纤维细胞，而它们对基质胶的黏附似乎是由特定机制介导的。未发现凝集素型相互作用参与这些细菌与底物的结合。用乳铁蛋白处理伴放线放线杆菌或成纤维细胞均可使黏附呈剂量依赖性降低，而单独用乳铁蛋白处理基质胶则没有抗黏附作用。中间普氏菌和变黑普氏菌对基质胶的黏附不受离子强度的显著影响，但乳铁蛋白的存在会抑制黏附。与基质胶、中间普氏菌和变黑普氏菌结合的乳铁蛋白会迅速释放，而与伴放线放线杆菌和成纤维细胞结合的乳铁蛋白则会保留。这些发现表明，乳铁蛋白依赖性抑制伴放线放线杆菌、中间普氏菌和变黑普氏菌对成纤维细胞和基质胶的黏附可能涉及乳铁蛋白与细菌和底物的结合。黏附降低可能是由于特异性黏附素-配体相互作用以及非特异性电荷依赖性相互作用均被阻断。
