Hirano Yasuyuki, Kuroda Kouichiro, Tamura Muneaki, Hayashi Kunio
Department of Bacteriology, Nihon University School of Dentistry, Tokyo 101-8310, Japan.
J Oral Sci. 2003 Jun;45(2):99-106. doi: 10.2334/josnusd.45.99.
Large quantities of Prevotella nigrescens ATCC 25261 (P. nigrescens) cells adhere to hydroxyapatite (HA) treated with extract from Porphyromonas gingivalis 381 (Pg-Ext), but not to HA coated with human serum albumin (HSA) or human serum globulin (HSG). The duration of HA treatment with Pg-Ext and several other conditions were tested to determine the factors causing Pg-Ext to promote P. nigrescens cell adhesion. Pg-Ext adsorbed rapidly to HA in less than 5 min. The maximum adherence of P. nigrescens cells to HA was observed after treatment of HSA and HSG and then retreatment of HA with Pg-Ext. It was found that Pg-Ext heated at 80 degrees C for 30 min did not lose its propensity to promote attachment of P. nigrescens to HA and that it also remained stable at 4 degrees C for at least 6 days. The trypsin-like enzyme activity of Pg-Ext was also measured, with BAPNA as the substrate and commercially purchased trypsin as the standard, and was approximately 0.12 units/mg. These data suggest that the presence of Pg-Ext is one of the essential factors responsible for P. nigrescens cell attachment to apatitic surfaces, and that with its trypsin-like activity, Pg-Ext may be considered an extremely important substance for the establishment of P. nigrescens in the periodontal pocket and the development of periodontal disease.
大量变黑普雷沃菌ATCC 25261（变黑普雷沃菌）细胞可黏附于用牙龈卟啉单胞菌381提取物（Pg-Ext）处理过的羟基磷灰石（HA），但不黏附于包被人血清白蛋白（HSA）或人血清球蛋白（HSG）的HA。测试了用Pg-Ext处理HA的持续时间及其他几种条件，以确定导致Pg-Ext促进变黑普雷沃菌细胞黏附的因素。Pg-Ext在不到5分钟内迅速吸附到HA上。在用HSA和HSG处理然后再用Pg-Ext处理HA后，观察到变黑普雷沃菌细胞对HA的最大黏附。发现80℃加热30分钟的Pg-Ext并未丧失促进变黑普雷沃菌黏附于HA的倾向，并且在4℃下至少6天仍保持稳定。还用BAPNA作为底物并以市售胰蛋白酶作为标准来测定Pg-Ext的类胰蛋白酶活性，其约为0.12单位/毫克。这些数据表明，Pg-Ext的存在是变黑普雷沃菌细胞黏附于磷灰石表面的重要因素之一，并且鉴于其类胰蛋白酶活性，Pg-Ext可能被认为是变黑普雷沃菌在牙周袋中定植及牙周疾病发展的极其重要的物质。
