Arata de Bellabarba G, Tortolero I, Villarroel V, Molina C Z, Bellabarba C, Velazquez E
Departamento de Fisiopatologia, Mérida, Venezuela.
Arch Androl. 2000 Nov-Dec;45(3):131-6. doi: 10.1080/01485010050193896.
The prevalence and clinical significance of leukocytes (WBC) and immature germ cells in semen is currently a matter of controversy. The aim of this work was to assess the prevalence of leukocytospermia in semen samples from Venezuelan men and its possible effects on sperm parameters. The concentration of WBC and round cells (RC) was evaluated in 118 semen samples from 19 fertile subjects (group 1), 62 infertile patients (group II), and 37 men with varicocele (group III). Semen WBC concentration was assessed by peroxidase assay. Twenty-six (22%) of the total samples had more than 10 WBC/mL semen. Twenty of the infertile men had leukocytospermia (32%) compared with 16% in the fertile group and 8% in the varicocele group. Semen RC concentration was lower than 5 x 10(6)/mL in all groups but, in groups II and III was significantly higher compared with group I. Infertile men had the highest WBC concentration. WBC concentration was negatively correlated with progressive motility, percentage of morphologically normal sperm, and hypoosmotic swelling test in infertile men but not in the varicocele group. In this group a negative correlation was obtained between immature germ cells and normal sperm morphology. The data show that leukcytospermia occurs frequently in infertile patients and is associated with poor semen quality parameters. In contrast, in men with varicocele, the increased number of immature germ cells might play a pivotal role in the pathogenesis of abnormal spermatozoa.
精液中白细胞（WBC）和未成熟生殖细胞的发生率及其临床意义目前存在争议。本研究的目的是评估委内瑞拉男性精液样本中白细胞精子症的发生率及其对精子参数的可能影响。对19名生育男性（第1组）、62名不育患者（第II组）和37名精索静脉曲张男性（第III组）的118份精液样本中的WBC和圆形细胞（RC）浓度进行了评估。通过过氧化物酶测定法评估精液WBC浓度。在所有样本中，26份（22%）精液中的WBC/mL超过10个。20名不育男性患有白细胞精子症（32%），而生育组为16%，精索静脉曲张组为8%。所有组的精液RC浓度均低于5×10⁶/mL，但第II组和第III组与第I组相比显著更高。不育男性的WBC浓度最高。在不育男性中，WBC浓度与进行性运动能力、形态正常精子百分比和低渗肿胀试验呈负相关，但在精索静脉曲张组中并非如此。在该组中，未成熟生殖细胞与正常精子形态之间呈负相关。数据表明，白细胞精子症在不育患者中频繁发生，并与精液质量参数差有关。相比之下，在精索静脉曲张男性中，未成熟生殖细胞数量的增加可能在异常精子发生的发病机制中起关键作用。
