Moubasher Alaa, Sayed Heba, Mosaad Eman, Mahmoud Ahmed, Farag Fawzy, Taha Emad A
Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Assiut University, Egypt.
Department of Clinical Pathology - South Egypt Cancer Institute, Assiut University, Egypt.
Cent European J Urol. 2018;71(4):470-475. doi: 10.5173/ceju.2018.1724. Epub 2018 Sep 27.
To characterize sperm dynamic motility patterns and chromatin integrity in infertile men with leukocytospermia.
Fifty patients with primary infertility and oligoasthenoteratozoospermia included in this prospective, controlled, blind study. All patients underwent clinical evaluation, semen peroxidase stain, computer aided semen analysis (CASA), sperm DNA integrity evaluation with acridine orange test (AOT) and fluorescence in situ hybridization (FISH) analysis of 18, X and Y chromosomes. Pregnancy outcomes were documented following antibiotic treatment of patients with leukocytospermia.
Infertile men with leukocytospermia had significantly lower progressive and total sperm motility percentages compared to the control group. Sperm dynamic motility parameters by CASA including curvilinear, straight line and average pathway velocities, straightness and amplitude of lateral head displacement were significantly lower in leukocytospermia. Sperm DNA fragmentation index was significantly higher in leukocytospermia. Percentages of sperm with disomy XY and 18 were significantly higher. These changes in sperm motility parameters and DNA integrity correlated with the number of peroxidase positive leukocytes. Follow-up of 23 of the 25 patients with leukocytospermia after antibiotic treatment revealed significantly higher pregnancy rates in cured patients than in those with persistent leukocytospermia.
Leukocytospermia has a significant impact on sperm dynamic motility patterns, DNA and chromosomal integrity in infertile men which can adversely affect the likelihood of a successful pregnancy.
对患有白细胞精子症的不育男性的精子动态运动模式和染色质完整性进行特征描述。
本前瞻性、对照、盲法研究纳入了50例原发性不育且患有少弱畸精子症的患者。所有患者均接受了临床评估、精液过氧化物酶染色、计算机辅助精液分析（CASA）、用吖啶橙试验（AOT）评估精子DNA完整性以及对18号、X和Y染色体进行荧光原位杂交（FISH）分析。对患有白细胞精子症的患者进行抗生素治疗后记录妊娠结局。
与对照组相比，患有白细胞精子症的不育男性的精子前向运动和总运动百分比显著更低。通过CASA检测的精子动态运动参数，包括曲线速度、直线速度和平均路径速度、直线性和头部侧向位移幅度，在白细胞精子症患者中显著更低。白细胞精子症患者的精子DNA碎片指数显著更高。XY和18号染色体二体的精子百分比显著更高。精子运动参数和DNA完整性的这些变化与过氧化物酶阳性白细胞的数量相关。对25例白细胞精子症患者中的23例进行抗生素治疗后的随访显示，治愈患者的妊娠率显著高于仍患有白细胞精子症的患者。
白细胞精子症对不育男性的精子动态运动模式、DNA和染色体完整性有显著影响，这可能对成功妊娠的可能性产生不利影响。
