van Riemsdijk I C, Baan C C, Knoop C J, Vantrimpont P J, Balk A H, Maat A P, Weimar W
Department of Internal Medicine 1, University Hospital Rotterdam-Dijkzigt, Rotterdam, The Netherlands.
Transpl Int. 2000;13 Suppl 1:S212-5. doi: 10.1007/s001470050327.
After heart transplantation a high incidence of infections and malignancies is found. Not only immunosuppression, but also intrinsic cytokine systems with some unbalance, e. g. TNF-alpha and IL-2, can result in impaired immune competence and may have a role in these complications. The aim of this study was to assess the activity of the TNF-alpha and IL-2 systems after heart transplantation. In peripheral blood we measured expression of activation markers of TNF-alpha (TNF-R2) and IL-2 (IL-2R alpha, IL-2R beta-chain) on monocytes and lymphocytes using quantitative flow-cytometric analysis. TNF-R2 expression was significantly enhanced on monocytes and lymphocytes in patients after heart transplantation, while the expression of IL-2R alpha and IL-2R beta was not elevated. Increased TNF-R2 expression in peripheral blood after heart transplantation reflects an activated TNF-alpha system, leading to high levels of active sTNF-R, which impairs TNF-alpha bioavailibility and consequently leads to immune incompetence.
心脏移植后，感染和恶性肿瘤的发生率很高。不仅免疫抑制，而且某些内在细胞因子系统失衡，例如肿瘤坏死因子-α（TNF-α）和白细胞介素-2（IL-2），都可能导致免疫能力受损，并可能在这些并发症中起作用。本研究的目的是评估心脏移植后TNF-α和IL-2系统的活性。我们使用定量流式细胞术分析，测量外周血中单核细胞和淋巴细胞上TNF-α（TNF-R2）和IL-2（IL-2Rα、IL-2Rβ链）激活标志物的表达。心脏移植患者的单核细胞和淋巴细胞上TNF-R2表达显著增强，而IL-2Rα和IL-2Rβ的表达并未升高。心脏移植后外周血中TNF-R2表达增加反映了TNF-α系统被激活，导致活性可溶性TNF-R水平升高，这损害了TNF-α的生物利用度，进而导致免疫无能。
