成纤维细胞培养中的缺氧。4. 1%氧气浓度下的细胞密度、葡萄糖利用及酸性黏多糖
Hypoxia in fibroblast cultures. 4. Cell density, glucose utilization and acid mucopolysaccharides in 1% O2 concentration.
作者信息
Kittlick P D
出版信息
Exp Pathol (Jena). 1977;13(4-5):217-25.
PMID:20320
Abstract
Secondary cultures of embryonic rat fibroblasts incubated in Eagle medium with Hepes buffer at pH 6.6 and 7.4 were maintained in hypoxia of 1% O2 for 48 hours. The acid mucopolysaccharides were isolated from medium plus cells and fractionated on cellulose microcolumns according to SVEJCAR and ROBERTSON (1967). Glucose utilization, lactate production, quantity of total MPS and changes in the MPS distribution pattern were correlated with cell density.
摘要
在含有Hepes缓冲液、pH值分别为6.6和7.4的伊格尔培养基中培养的胚胎大鼠成纤维细胞的传代培养物，在1%氧气的低氧环境中维持48小时。从培养基和细胞中分离出酸性粘多糖，并根据斯韦贾卡尔和罗伯逊（1967年）的方法在纤维素微柱上进行分级分离。葡萄糖利用、乳酸生成、总粘多糖的量以及粘多糖分布模式的变化与细胞密度相关。
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