Telegdy G, Adamik A, Glover V
Institute of Pathophysiology, University of Szeged, Szeged, Hungary.
Brain Res Bull. 2000 Oct;53(3):367-70. doi: 10.1016/s0361-9230(00)00359-2.
Isatin is an endogenous indole which has been shown to counteract some of the effects of atrial natriuretic peptide (ANP) both in vitro and in vivo. The present study was designed to determine whether it could antagonise in vivo effects of the related peptides brain natriuretic peptide (BNP) and C-type natriuretic peptide (CNP). The model used was consolidation of memory in a one-trial step-through passive-avoidance paradigm in the rat. Previous studies have shown that all three peptides (1 microg intracerebroventricular) can consolidate such learning and increase latency to entry a dark box. Isatin was given intraperitoneally at doses of 5, 10 and 50 mg/kg before the peptide, or a saline control. Both BNP and CNP significantly increased the latency of entry. Isatin alone had no effect. Isatin reduced the effect of both BNP and CNP; this was significant for its effect on BNP at 50 mg/kg and on CNP at both 10 and 50 mg/kg. These results show that isatin can inhibit behavioural effects of BNP and CNP as well as ANP.
异吲哚酮是一种内源性吲哚，已被证明在体外和体内均可对抗心房利钠肽（ANP）的某些作用。本研究旨在确定它是否能拮抗相关肽类脑利钠肽（BNP）和C型利钠肽（CNP）的体内作用。所使用的模型是大鼠单次步入式被动回避范式中的记忆巩固。先前的研究表明，所有这三种肽（脑室内注射1微克）均可巩固此类学习并增加进入暗箱的潜伏期。在注射肽之前，以5、10和50毫克/千克的剂量腹腔注射异吲哚酮，或注射生理盐水作为对照。BNP和CNP均显著增加了进入潜伏期。单独使用异吲哚酮没有效果。异吲哚酮可减弱BNP和CNP的作用；其对50毫克/千克的BNP以及10和50毫克/千克的CNP的作用具有显著性。这些结果表明，异吲哚酮可抑制BNP、CNP以及ANP的行为学作用。
