Lundy F T, Chalk R, Lamey P J, Shaw C, Linden G J
School of Dentistry, The Queen's University of Belfast, Belfast, UK.
J Pathol. 2000 Dec;192(4):540-4. doi: 10.1002/1096-9896(2000)9999:9999<::AID-PATH740>3.0.CO;2-1.
The purpose of the study was to analyse how the protein composition of the inflammatory exudate associated with chronic periodontitis differed from the exudate in periodontal health. Gingival crevicular fluid (GCF) was collected from sites with chronic periodontal inflammation and from non-diseased sites in healthy control subjects. Microbore HPLC analysis revealed one major difference in GCF protein profiles between healthy controls and periodontitis patients. The protein enhanced in periodontitis patients was identified as migration inhibitory factor-related protein-8 (MRP-8) by a combination of N-terminal amino acid sequencing, mass spectrometry, and SDS-PAGE. Together, these data demonstrate, for the first time, the presence of monomeric MRP-8 in an inflammatory exudate. Whether monomeric MRP-8 is a unique feature of chronic periodontal inflammation is not yet clear, but the chemotactic properties of this peptide support a functional role for MRP-8 in periodontal inflammation.
本研究的目的是分析与慢性牙周炎相关的炎症渗出物的蛋白质组成与牙周健康状态下的渗出物有何不同。从患有慢性牙周炎症的部位以及健康对照受试者的非患病部位收集龈沟液（GCF）。微孔高效液相色谱分析显示，健康对照者和牙周炎患者的GCF蛋白质谱存在一个主要差异。通过N端氨基酸测序、质谱分析和SDS-PAGE相结合的方法，确定牙周炎患者中增强的蛋白质为迁移抑制因子相关蛋白8（MRP-8）。这些数据首次共同证明了炎症渗出物中存在单体MRP-8。单体MRP-8是否是慢性牙周炎症的独特特征尚不清楚，但该肽的趋化特性支持MRP-8在牙周炎症中发挥功能作用。
