[Electrophoresis of proteins in gingival crevicular fluid of patients with adult periodontitis before and after treatment].

OBJECTIVE

To analyze the overall gingival crevicular fluid (GCF) protein profiles from adult periodontitis (AP) both before and one month after conventional periodontal treatment in expectation to find reliable specific protein markers in GCF for healthy and disease.

METHODS

GCF samples were collected using filter paper strips from 56 sites of 12 AP patients. The proteins of GCF were examined via sodium dodecyle sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and analyzed by the Image Master Electrophoresis Evaluation System.

RESULTS

After periodontal treatment, the mean number of protein bands and the abundance and percentage of non-serum derived low molecular weight proteins (12,000, 10,000) decreased significantly (P < 0.001 and < 0.05 respectively). The percentage of non-serum derived 38,000 protein increased and correlated negatively with pocket depth and the volume of GCF before treatment.

CONCLUSION

1. the elevation of the 12,000 and 10,000 proteins are associated with the periodontal inflammation; 2. the elevation of 38,000 protein is associated with recovery.