Zhou H, Meng H, Chen Z
School of Stomatology, Beijing Medical University, Beijing 100081.
Zhonghua Kou Qiang Yi Xue Za Zhi. 1998 Mar;33(2):109-12.
To seek the protein composition related to periodontal diseases.
86 gingival crevicular fluid (GCF) samples were collected by rinsing the sulgingival sulci from subjects with healthy gingiva (20), gingivitis (24) and periodontitis (42). The protein composition were examined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) on a Phast System. The gels were scanned and analyzed using Phast Image with computer.
In inflammatory condition, the serum derived proteins with MW 82,000, 77,000, 17,000 occurred more frequently than in healthy gingiva, and the occurrence and abundance of non-serum derived proteins with MW 41,000, 21,000, 12,000 were also increased in inflammatory condition compared with healthy gingiva.
The high levels of proteins with MW 82,000, 41,000, 21,000, 12,000 are close related to the periodontal inflammatory status.
探寻与牙周疾病相关的蛋白质组成。
从健康牙龈（20例）、牙龈炎（24例）和牙周炎（42例）受试者的龈沟中冲洗收集86份龈沟液（GCF）样本。蛋白质组成通过在Phast系统上进行十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）检测。凝胶用Phast Image进行扫描并用计算机分析。
在炎症状态下，分子量为82,000、77,000、17,000的血清源性蛋白质出现频率高于健康牙龈，与健康牙龈相比，分子量为41,000、21,000、12,000的非血清源性蛋白质的出现及丰度在炎症状态下也增加。
分子量为82,000、41,000、21,000、12,000的蛋白质水平与牙周炎症状态密切相关。
