Barnes I, Holton J, Vaira D, Spigelman M, Thomas M G
Department of Bacteriology, The Windeyer Institute of Medical Sciences, The Windeyer Building, 46 Cleveland Street, London W1P 6DB, UK.
J Pathol. 2000 Dec;192(4):554-9. doi: 10.1002/1096-9896(2000)9999:9999<::AID-PATH768>3.0.CO;2-C.
To examine the potential for DNA recovery from spirit-preserved medical material, a set of specimens from the Hunterian Collection of the Royal College of Surgeons was investigated. Using a range of DNA extraction techniques and the PCR, no replicable positive amplifications were made from this material of either human or Helicobacter DNA. Experiments with modern stomach biopsies of H. pylori-positive patients suggest that the bacterial DNA is typically present in a much lower concentration (10(3)-fold) than that of the host. The potential for recovery of this organism from spirit specimens is therefore low. The absence of DNA in this material is probably due to several factors, chiefly the incomplete fixation of the specimen by the ethanol storage fluid. Studies such as this demonstrate the need for a good understanding of specimen history when working with archival material.
为了研究从用酒精保存的医学材料中回收DNA的可能性，对皇家外科医学院亨特博物馆收藏的一组标本进行了调查。使用一系列DNA提取技术和聚合酶链反应（PCR），从该材料中未获得人类或幽门螺杆菌DNA的可重复阳性扩增产物。对幽门螺杆菌阳性患者的现代胃活检样本进行的实验表明，细菌DNA的浓度通常比宿主DNA低得多（低10³倍）。因此，从酒精保存的标本中回收这种微生物的可能性较低。该材料中没有DNA可能是由于几个因素，主要是乙醇保存液对标本的固定不完全。此类研究表明，在处理档案材料时，需要充分了解标本的历史。
