Dawidowicz A L, Fornal E
Department of Chemical Physics and Physicochemical Separation Methods, Faculty of Chemistry, Maria Curie-Sklodowska University, 20-031 Lublin, Pl. M. Curie-Sklodowskiej 3, Poland.
Biomed Chromatogr. 2000 Nov;14(7):493-7. doi: 10.1002/1099-0801(200011)14:7<493::AID-BMC999>3.0.CO;2-T.
Propofol (2,6-diisopropylphenol) is a short-acting drug with a large volume of distribution and high body clearance. It is suitable both for the induction of anaesthesia by bolus injection and the maintenance of anaesthesia by repeated injections or a continuous infusion. Examining the drug concentration its analysis in whole blood is recommended. This results from the fact that propofol molecules strongly bind with plasma proteins and cellular blood constituents and blood composition variations are observed between individuals or in different disease states or resulting from transfusion etc. In most cases the HPLC analysis follows the extraction of samples. The degree of propofol binding with blood cells can be different, depending on the blood type, and it can change in time, which may affect the results of the analysis. The paper discusses and shows the necessity of blood cell lysis before the extraction procedure. The cell lysis makes possible to determine the total amount of propofol in blood independently of the degree of propofol binding with cellular blood constituents and its changes.
丙泊酚（2,6 - 二异丙基苯酚）是一种作用迅速的药物，分布容积大，机体清除率高。它既适用于单次静脉注射诱导麻醉，也适用于重复注射或持续输注维持麻醉。检测药物浓度时，建议分析全血中的药物浓度。这是因为丙泊酚分子与血浆蛋白和血细胞成分紧密结合，个体之间、不同疾病状态下或输血等情况后会观察到血液成分的变化。在大多数情况下，高效液相色谱分析是在样品提取之后进行。丙泊酚与血细胞的结合程度可能因血型而异，并且会随时间变化，这可能会影响分析结果。本文讨论并表明了在提取步骤之前进行血细胞裂解的必要性。血细胞裂解能够独立于丙泊酚与血细胞成分的结合程度及其变化来测定血液中丙泊酚的总量。
