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增强基质辅助激光解吸/电离质谱中赖氨酸末端胰蛋白酶肽离子的强度。

Enhancing the intensities of lysine-terminated tryptic peptide ions in matrix-assisted laser desorption/ionization mass spectrometry.

作者信息

Beardsley R L, Karty J A, Reilly J P

机构信息

Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.

出版信息

Rapid Commun Mass Spectrom. 2000;14(23):2147-53. doi: 10.1002/1097-0231(20001215)14:23<2147::AID-RCM145>3.0.CO;2-M.

DOI:10.1002/1097-0231(20001215)14:23<2147::AID-RCM145>3.0.CO;2-M
PMID:11114023
Abstract

Tryptic digests of three proteins are reacted with O-methylisourea in order to convert lysine residues to homoarginines. The resulting homoarginine-terminated peptides exhibit more intense MALDI mass spectral peaks than their lysine-terminated predecessors. This simple chemical reaction should therefore facilitate protein sequencing and mass mapping.

摘要

三种蛋白质的胰蛋白酶消化产物与O-甲基异脲反应，以便将赖氨酸残基转化为高精氨酸。由此产生的以高精氨酸结尾的肽段，其基质辅助激光解吸电离质谱峰比以赖氨酸结尾的前身肽段更强烈。因此，这个简单的化学反应应有助于蛋白质测序和质谱图谱分析。

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