Li T Y, Liu B H, Chen Y C
Institute of Toxicology, Tzu Chi University, Hualien 970, Taiwan.
Rapid Commun Mass Spectrom. 2000;14(24):2393-400. doi: 10.1002/1097-0231(20001230)14:24<2393::AID-RCM178>3.0.CO;2-9.
The intact fungal spores of several strains of four Aspergillus species, Aspergillus flavus, A. oryzae, A. parasiticus, and A. sojae, were directly analyzed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry. Very simple MALDI mass spectra are obtained by directly mixing spores with a matrix such as alpha-cyano-4-hydroxycinnamic acid or sinapinic acid. The mass spectra are obtained from the ablation of cell walls of spores owing to the acidity of the matrix solution. The MALDI results show that aflatoxigenic strains and non-aflatoxigenic strains have different mass peak profiles. Furthermore, the MALDI results of non-aflatoxigenic A. flavus and A. parasiticus spores resemble those of the closely related A. oryzae and A. sojae spores, respectively.
利用基质辅助激光解吸/电离（MALDI）飞行时间质谱直接分析了黄曲霉、米曲霉、寄生曲霉和酱油曲霉这四种曲霉菌株的完整真菌孢子。将孢子与α-氰基-4-羟基肉桂酸或芥子酸等基质直接混合，即可获得非常简单的MALDI质谱。由于基质溶液的酸性，通过孢子细胞壁的烧蚀获得质谱。MALDI结果表明，产黄曲霉毒素菌株和不产黄曲霉毒素菌株具有不同的质谱峰谱。此外，不产黄曲霉毒素的黄曲霉和寄生曲霉孢子的MALDI结果分别与密切相关的米曲霉和酱油曲霉孢子的结果相似。
