Wilkendorf Laura S, Bowles Edmée, Buil Jochem B, van der Lee Henrich A L, Posteraro Brunella, Sanguinetti Maurizio, Verweij Paul E
Radboud University Medical Center, Department of Medical Microbiology, Nijmegen, the Netherlands.
Radboud University Medical Center, Department of Medical Microbiology, Nijmegen, the Netherlands
J Clin Microbiol. 2020 Nov 18;58(12). doi: 10.1128/JCM.01263-20.
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based species identification has found its place in many clinical routine diagnostic laboratories over the past years, allowing significantly reduced turnaround times and high-precision results. With regard to MALDI-TOF MS for filamentous fungi, here, we discuss different approaches for sample processing and growth conditions before analysis. In particular, we review the performances of different commercially available databases as well as the potential of complementary (self-constructed) in-house databases.
在过去几年中，基于基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）的菌种鉴定已在许多临床常规诊断实验室中占据一席之地，可显著缩短周转时间并获得高精度结果。关于丝状真菌的MALDI-TOF MS，在此我们讨论分析前样品处理和生长条件的不同方法。特别是，我们回顾了不同商业可用数据库的性能以及互补（自建）内部数据库的潜力。
