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1
Evaluation of ID Fungi Plates Medium for Identification of Molds by MALDI Biotyper.用于通过基质辅助激光解吸电离生物分型仪鉴定霉菌的ID真菌平板培养基的评估
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2
Multicenter Study Demonstrates Standardization Requirements for Mold Identification by MALDI-TOF MS.多中心研究证明了基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定霉菌的标准化要求。
Front Microbiol. 2019 Sep 20;10:2098. doi: 10.3389/fmicb.2019.02098. eCollection 2019.
3
Vitek MS v3.0 System in the Identification of Filamentous Fungi.Vitek MS v3.0 系统在丝状真菌鉴定中的应用。
Mycopathologia. 2019 Oct;184(5):645-651. doi: 10.1007/s11046-019-00377-0. Epub 2019 Sep 10.
4
Multi-centric evaluation of the online MSI platform for the identification of cryptic and rare species of Aspergillus by MALDI-TOF.通过 MALDI-TOF 对 Aspergillus 隐种和稀有种进行在线 MSI 平台的多中心评估。
Med Mycol. 2019 Nov 1;57(8):962-968. doi: 10.1093/mmy/myz004.
5
MALDI-TOF MS-Based Identification of Melanized Fungi is Faster and Reliable After the Expansion of In-House Database.基于 MALDI-TOF MS 的黑色素真菌鉴定在扩展内部数据库后更快、更可靠。
Proteomics Clin Appl. 2019 May;13(3):e1800070. doi: 10.1002/prca.201800070. Epub 2018 Sep 24.
6
Evaluation of three MALDI-TOF mass spectrometry libraries for the identification of filamentous fungi in three clinical microbiology laboratories in Manitoba, Canada.评价三种 MALDI-TOF 质谱文库在加拿大马尼托巴省三个临床微生物学实验室鉴定丝状真菌的效果。
Mycoses. 2018 Oct;61(10):743-753. doi: 10.1111/myc.12800. Epub 2018 Jul 2.
7
Increased species-assignment of filamentous fungi using MALDI-TOF MS coupled with a simplified sample processing and an in-house library.使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）结合简化的样品处理和内部数据库提高丝状真菌的物种鉴定率。
Med Mycol. 2019 Jan 1;57(1):63-70. doi: 10.1093/mmy/myx154.
8
Species Identification and Delineation of Pathogenic Mucorales by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.基质辅助激光解吸电离飞行时间质谱法鉴定和区分致病性毛霉目真菌。
J Clin Microbiol. 2018 Mar 26;56(4). doi: 10.1128/JCM.01886-17. Print 2018 Apr.
9
Identification of clinical isolates of Aspergillus, including cryptic species, by matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定曲霉菌临床分离株，包括隐性种。
Med Mycol. 2018 Oct 1;56(7):838-846. doi: 10.1093/mmy/myx115.
10
Multicenter Evaluation of the Vitek MS v3.0 System for the Identification of Filamentous Fungi.多中心评价 Vitek MS v3.0 系统用于鉴定丝状真菌。
J Clin Microbiol. 2018 Jan 24;56(2). doi: 10.1128/JCM.01353-17. Print 2018 Feb.

基质辅助激光解吸电离飞行时间质谱法鉴定丝状真菌的研究进展

Update on Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Identification of Filamentous Fungi.

作者信息

Wilkendorf Laura S, Bowles Edmée, Buil Jochem B, van der Lee Henrich A L, Posteraro Brunella, Sanguinetti Maurizio, Verweij Paul E

机构信息

Radboud University Medical Center, Department of Medical Microbiology, Nijmegen, the Netherlands.

Radboud University Medical Center, Department of Medical Microbiology, Nijmegen, the Netherlands

出版信息

J Clin Microbiol. 2020 Nov 18;58(12). doi: 10.1128/JCM.01263-20.

DOI:10.1128/JCM.01263-20
PMID:32938733
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7685878/
Abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based species identification has found its place in many clinical routine diagnostic laboratories over the past years, allowing significantly reduced turnaround times and high-precision results. With regard to MALDI-TOF MS for filamentous fungi, here, we discuss different approaches for sample processing and growth conditions before analysis. In particular, we review the performances of different commercially available databases as well as the potential of complementary (self-constructed) in-house databases.

摘要

在过去几年中，基于基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）的菌种鉴定已在许多临床常规诊断实验室中占据一席之地，可显著缩短周转时间并获得高精度结果。关于丝状真菌的MALDI-TOF MS，在此我们讨论分析前样品处理和生长条件的不同方法。特别是，我们回顾了不同商业可用数据库的性能以及互补（自建）内部数据库的潜力。