Zavalin Andre, Yang Junhai, Hayden Kevin, Vestal Marvin, Caprioli Richard M
The Mass Spectrometry Research Center and Department of Biochemistry, Vanderbilt University, Nashville, TN, 37235, USA.
Anal Bioanal Chem. 2015 Mar;407(8):2337-42. doi: 10.1007/s00216-015-8532-6. Epub 2015 Feb 12.
We have achieved protein imaging mass spectrometry capabilities at sub-cellular spatial resolution and at high acquisition speed by integrating a transmission geometry ion source with time of flight mass spectrometry. The transmission geometry principle allowed us to achieve a 1-μm laser spot diameter on target. A minimal raster step size of the instrument was 2.5 μm. Use of 2,5-dihydroxyacetophenone robotically sprayed on top of a tissue sample as a matrix together with additional sample preparation steps resulted in single pixel mass spectra from mouse cerebellum tissue sections having more than 20 peaks in a range 3-22 kDa. Mass spectrometry images were acquired in a standard step raster microprobe mode at 5 pixels/s and in a continuous raster mode at 40 pixels/s.
通过将透射式离子源与飞行时间质谱联用，我们实现了亚细胞空间分辨率和高采集速度的蛋白质成像质谱分析能力。透射式原理使我们能够在目标上实现1μm的激光光斑直径。该仪器的最小光栅步长为2.5μm。将2,5 - 二羟基苯乙酮自动喷在组织样品上作为基质，并结合其他样品制备步骤，从小鼠小脑组织切片中获得了单像素质谱图，在3 - 22 kDa范围内有超过20个峰。质谱图像以标准步长光栅微探针模式以5像素/秒的速度采集，以及以连续光栅模式以40像素/秒的速度采集。
